首頁 > 網路資源 > 大同大學數位論文系統

Title page for etd-0114110-004357


URN etd-0114110-004357 Statistics This thesis had been viewed 3104 times. Download 2273 times.
Author Hung-che Chou
Author's Email Address No Public.
Department Bioengineering
Year 2009 Semester 1
Degree Ph.D. Type of Document Doctoral Dissertation
Language zh-TW.Big5 Chinese Page Count 174
Title USING QUARTZ CRYSTAL MICROBALANCE TO DEVELOP THE CELL DETECTION PLATFORM
Keyword
  • CELL DETECTION
  • QUARTZ CRYSTAL MICROBALANCE
  • QUARTZ CRYSTAL MICROBALANCE
  • CELL DETECTION
  • Abstract A cell detecting system was fabricated in this study. The system includes three major parts. One is the cell incubator chamber, another is the QCM sensor chip, and the other is the cell circuit system. The frequency is decreased with more cells growing on QCM chip. The result indicates that when cell grow on the chip the cell membrane adhered on the surface of electrode and produced higher density and viscosity, and it made the decrease of frequency. When cells were full of chip surface, we added trypsin-EDTA solution inducing the cells leaved chip surface. Thus, the frequency change was increased. After we confirmed the biocompatibility by cell survival experiment, using NH4OH and H2O2 solution to increase the surface hydrophilic can help cell adhere on chip surface.
    We used the cell detecting system to estimate the completeness of cell tight junction. The completeness of cell tight junction would be detected by using different solution in QCM system. We choose Caco-2 cell as test cell line, and detected its growth condition when Caco-2 cells grew on the QCM chip. The result showed that the QCM resonance frequency changes less when a better tight cell junction is formed. The cell tight junction integrality is completed up to 90% when the change of frequency was less than 100Hz. We proved the possibility of detecting Caco-2 cell tight junction by using QCM system.
    We established a membrane process by plasma treatment and graft polymerization to form a protection film to protect QCM sliver electrode. After establishing the process condition of HMDSZ plasma film, we choose one of three ways to increase the chip hydrophilic ability. For overall concerned with water contact angle test, cell survival experiment and electrode protective analysis, it suggested graft polymerization AAm be the better effect. We proved that using improved silver electrode QCM with a protection film can detect cell growth of Caco-2, and believed that this system can be used for detection of other kind cell growth.
    Advisor Committee
  • Tsong-rong Yan - advisor
  • none - co-chair
  • none - co-chair
  • none - co-chair
  • none - co-chair
  • none - co-chair
  • Files indicate in-campus access immediately and off-campus access at 2 years
    Date of Defense 2009-12-04 Date of Submission 2010-01-14


    Browse | Search All Available ETDs