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Title page for etd-0124114-132900


URN etd-0124114-132900 Statistics This thesis had been viewed 842 times. Download 1 times.
Author Chung-yi Huang
Author's Email Address No Public.
Department Bioengineering
Year 2013 Semester 1
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 69
Title THE PORDUCTION PROCEDURE INVESTGATION OF LACTONE TYPE RAPAMYCIN
Keyword
  • Rapamycin
  • Sirolimus
  • Sirolimus
  • Rapamycin
  • Abstract Rapamycin is a new natural macrocyclic lactone of immunosuppressant for preventing organ transplant rejection, and is also known as antifungal and anti-tumor drug. Rapamycin and its relative chemical derivatives, e.g. temsirolimus, everolimus, and dimethyl-phosphinic acid C-43 rapamycin ester, could complex with the mammalian target of rapamycin (mTOR) that is responsible for regulating cell growth and proliferation, and could be functioned as inhibiting tumor growth or inducing cancer cell apoptosis. Due to the high commercial benefit of rapamycin, it is worth to study the fermentation technique of increasing the production quantity of rapamycin and their effecting factors. In this study, we investigated the multiple effecting factors that influence the commercial fermentation process of rapamycin. The checking of fermentation devices and key steps in production procedure, e.g. detail operational procedure and management of raw materials, etc were investigated. We found that contaminant problem of rapamycin producing strain in the stocking vial could be a key factor effecting the production quantity of rapamycin. Due the low level of such contamination and the rare vials exposed to the contamination, it is difficult to find this problem easily. Besides, due to the strong biological activity of rapamycin producing strain, it could digest part of the contaminant strain, and resulted in low detection of contaminant strain that will significantly affect the productivity of rapamycin at the end of fermentation. Solving the above problem of contamination resulted in the achievement of high productivity of rapamycin in this study.
    Advisor Committee
  • C. Will Chen - advisor
  • Dey-Chyi Sheu - co-chair
  • JI-CAI LIN - co-chair
  • Files indicate in-campus access at one year and off-campus not accessible
    Date of Defense 2014-01-17 Date of Submission 2014-02-08


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