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Title page for etd-0125111-190839


URN etd-0125111-190839 Statistics This thesis had been viewed 2371 times. Download 0 times.
Author Shih-Hsiung Sung
Author's Email Address No Public.
Department Bioengineering
Year 2010 Semester 1
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 57
Title The Screening of Yeasts That Do Not Utilize Sucrose, Maltose or Lactose
Keyword
  • yeast
  • oligosaccharides
  • oligosaccharides
  • yeast
  • Abstract The low-content oligosaccharides including fructooligisaccharides (FOS), isomaltooligosaccharides (IMO) and galactooligosaccharides (GOS) consisted of not only oligosaccharides but also large amount of glucose and unreacted substrate sugars, such as sucrose, maltose and lactose. In order to eliminate these digestible sugars from oligosaccharides syrups and to increase the yields of oligosaccharides, selected yeasts were employed during the enzymatic reactions for the production of oligosaccharides. These yeasts should consume glucose alone but not the substrate sugars. During the oligosaccharides-forming reaction catalyzed by enzyme and yeast, the byproducts glucose was concurrently eliminated by yeast fermentation. This work was engaged in the screening of such yeasts that can ferment glucose but not sucrose, maltose or lactose. Yeasts were cultured in the fermentation medium composed of yeast extract, glucose and sucrose, maltose or lactose. Then the sugars in the fermented broth were analyzed by HPLC. Among four yeast strains, Wickerhamomyces anomala BCRC 20858 and Candida utilis BCRC 21990 could utilize sucrose but not maltose and lactose and Pichia bimundalis BCRC 20479 consume lactose slightly, whereas Wickerhamomyces anomalus BCRC 20866 did not utilize three sugars at all.
    Advisor Committee
  • Dey-Chyi Sheu - advisor
  • Files indicate not accessible
    Date of Defense 2011-01-20 Date of Submission 2011-01-26


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