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URN etd-0127114-140416 Statistics This thesis had been viewed 1735 times. Download 589 times. Author Ming-jyun Song Author's Email Address No Public. Department Bioengineering Year 2013 Semester 1 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 49 Title Study on cellulose production from four strains of Aspergillus sp. in liquid state fermentation Keyword Endoglucanase β-glucosdase Filter Paper Assay cellulose Aspergillus Aspergillus cellulose Filter Paper Assay β-glucosdase Endoglucanase Abstract The production of cellulose degrading enzymes by cultivation of Trichoderma reesei RUT C30, Botrytis cinerea BCRC 33112, Cordyceps militarisATCC 34164, Aspergillus niger BCRC 32720 was studied. Wheat bran suspension supplemented with α-cellulose hydrolyzate and yeast extract or peptone was used as cultivation medium. It was found that FPU(Filter Paper Assay), β-glucosdase and CMCase activities by Asp. niger 32720 were enhanced in the range of 28~35 % for the medium using wheat bran supplemented with 1 % α-cellulose hydrolyzate. α-Cellulose hydrolyzate performs as an inducer.
Wheat bran (10 %) supplemented with 0.5 % peptone and 1 % α-cellulose hydrolyzate in the medium was used to cultivate the 4 fungi to produce cellulase. The cultivation with B. cinerea BCRC 33112 gave the most activity of FPU (0.68 Unit/ml), β-glucosidase (0.65 Unit/ml), CMCase (0.58 Unit/ml). Trichoderma reesei RUT C30 gave the lowest activity among the four strains.
The experiment was duplicated by replacing 0.5 % peptone to 0.5 % yeast extract. B. cinerea BCRC 33112 again gave the most activity of FPU (0.78 Unit/ml), β-glucosidase (0.7 Unit/ml), CMCase (0.75 Unit/ml). Asp. niger BCRC 32720 ran the second most activity.
Advisor Committee Kow-Jen Duan - advisor
C. Will Chen - co-chair
CI-CAI Lin - co-chair
Files Date of Defense 2013-12-24 Date of Submission 2014-01-27