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Title page for etd-0127114-140416


URN etd-0127114-140416 Statistics This thesis had been viewed 1735 times. Download 589 times.
Author Ming-jyun Song
Author's Email Address No Public.
Department Bioengineering
Year 2013 Semester 1
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 49
Title Study on cellulose production from four strains of Aspergillus sp. in liquid state fermentation
Keyword
  • Endoglucanase
  • β-glucosdase
  • Filter Paper Assay
  • cellulose
  • Aspergillus
  • Aspergillus
  • cellulose
  • Filter Paper Assay
  • β-glucosdase
  • Endoglucanase
  • Abstract The production of cellulose degrading enzymes by cultivation of Trichoderma reesei RUT C30, Botrytis cinerea BCRC 33112, Cordyceps militarisATCC 34164, Aspergillus niger BCRC 32720 was studied. Wheat bran suspension supplemented with α-cellulose hydrolyzate and yeast extract or peptone was used as cultivation medium. It was found that FPU(Filter Paper Assay), β-glucosdase and CMCase activities by Asp. niger 32720 were enhanced in the range of 28~35 % for the medium using wheat bran supplemented with 1 % α-cellulose hydrolyzate. α-Cellulose hydrolyzate performs as an inducer.
       Wheat bran (10 %) supplemented with 0.5 % peptone and 1 % α-cellulose hydrolyzate in the medium was used to cultivate the 4 fungi to produce cellulase. The cultivation with B. cinerea BCRC 33112 gave the most activity of FPU (0.68 Unit/ml), β-glucosidase (0.65 Unit/ml), CMCase (0.58 Unit/ml). Trichoderma reesei RUT C30 gave the lowest activity among the four strains.
       The experiment was duplicated by replacing 0.5 % peptone to 0.5 % yeast extract. B. cinerea BCRC 33112 again gave the most activity of FPU (0.78 Unit/ml), β-glucosidase (0.7 Unit/ml), CMCase (0.75 Unit/ml). Asp. niger BCRC 32720 ran the second most activity.
    Advisor Committee
  • Kow-Jen Duan - advisor
  • C. Will Chen - co-chair
  • CI-CAI Lin - co-chair
  • Files indicate access worldwide
    Date of Defense 2013-12-24 Date of Submission 2014-01-27


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