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Title page for etd-0202109-115422


URN etd-0202109-115422 Statistics This thesis had been viewed 4015 times. Download 1283 times.
Author Hao-Chieh Hsieh
Author's Email Address No Public.
Department Bioengineering
Year 2008 Semester 1
Degree Master Type of Document Master's Thesis
Language English Page Count 100
Title Stabilization of D-amino acid oxidase from Rhodosporidium toruloides by immobilization onto magnetic nanoparticles
Keyword
  • Rhodosporidium toruloides
  • Magnetic nanoparticle
  • D-Amino acid oxidase
  • Stabilization
  • Immobilization
  • Immobilization
  • Stabilization
  • D-Amino acid oxidase
  • Magnetic nanoparticle
  • Rhodosporidium toruloides
  • Abstract D-Amino acid oxidase from Rhodosporidium toruloides was expressed by E. coli and immobilized onto glutaraldehyde activated magnetic nanoparticles. Approximately four enzyme molecules were attached to one magnetic nanoparticle when the weight ratio of the enzyme to the support was 0.12. After immobilization, the Tm increased from 45oC of the free form to 55oC, indicating the thermal stability was improved. At the presence of 20 mM hydrogen peroxide, the immobilized form retained 93% of its activity after 300 min while the free form was completely inactivated after 210 min, suggesting the oxidative stability against hydrogen peroxide was also improved. Although the stability was enhanced through immobilization, the catalytic efficiency (kcat/KM) was decreased by 3.5 fold. The immobilized enzyme could be recycled, 15% of the initial activity was retained after 10 reaction cycles.
    Advisor Committee
  • Chi-Yang Yu - advisor
  • I-Ching Kuan - co-chair
  • Ming-Kai Chern - co-chair
  • Files indicate access worldwide
    Date of Defense 2009-01-06 Date of Submission 2009-02-05


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