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Title page for etd-0215106-130431


URN etd-0215106-130431 Statistics This thesis had been viewed 1768 times. Download 17 times.
Author Hsing-Chun Hsieh
Author's Email Address No Public.
Department Bioengineering
Year 2005 Semester 2
Degree Master Type of Document Master's Thesis
Language English Page Count 64
Title THE RHIZOME TISSUE CULTURE AND ANALYSIS OF ANTIOXIDATION PROPERTY AND TECTORIGENIN OF NEOMARICA GRACILIS
Keyword
  • TECTORIGENIN
  • RHIZOME TISSUE CULTURE
  • NEOMARICA GRACILIS
  • ANTIOXIDATION PROPERTY
  • ANTIOXIDATION PROPERTY
  • NEOMARICA GRACILIS
  • RHIZOME TISSUE CULTURE
  • TECTORIGENIN
  • Abstract Rhizomes were induced from in vitro seedlings of Neomarica gracilis cultured in MS basal medium contained with 30 g/l sucrose, 0.5 mg/l BA, 0.84 mg/l NAA and 0.1 mg/l 2,4-D. Fast growing tissue obtained from the rhizome tissues were subcultured in the same liquid medium and the multiplication of rhizome tissues were 4-fold after 30 days culture. The influence of jasmonic acid, l-phenylalanine and yeast extract addition on the radical scavenging activity, total flavonoid amount and tectorigenin accumulation in rhizome tissues were investigated.
    The radical scavenging activity under system concentration 5 mg DW/ml can increase to 90 % in methanol extract from rhizome tissues subcultured in MS basal medium contained with 30 g/l sucrose, 0.5 mg/l BA, 0.84 mg/l NAA, 0.1 mg/l 2,4-D and yeast extract (100 ~ 400 mg/l).
    The total flavonoid amount and tectorigenin accumulation in cell increased in methanol extract from rhizome tissues subcultured in MS basal medium contained l-phenylalanine. The maximum total flavonoid reached 1.059 % DW, when rhizome tissues subcultured in MS basal medium contained with 30 g/l sucrose, 0.5 mg/l BA, 0.84 mg/l NAA, 0.1 mg/l 2,4-D and 300 mg/l l-phenylalanine. The rhizome tissues subcultured in MS basal medium contained with 30 g/l sucrose, 0.5 mg/l BA, 0.84 mg/l NAA, 0.1 mg/l 2,4-D and 200 mg/l l-phenylalanine has the highest tectorigenin content (0.00427 % DW). The rhizome of Neomarica gracilis planted in field is unable to measure the tectorigenin.
    Advisor Committee
  • Chin-Wen Ho - advisor
  • none - co-chair
  • none - co-chair
  • Files indicate in-campus access only
    Date of Defense 2006-01-05 Date of Submission 2006-02-15


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