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Title page for etd-0215111-141114


URN etd-0215111-141114 Statistics This thesis had been viewed 1984 times. Download 0 times.
Author Chin-Pin Hse
Author's Email Address 0915576828@yaoo.com.tw
Department Bioengineering
Year 2010 Semester 1
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 190
Title QUANTITATIVE METHODS TO EVALUATE THE ADENOVIRUS AND THE APPLICATION OF ENVIRONMENTAL WATER TESTING
Keyword
  • none
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  • Abstract In this study, there are two new quantitative methods Integrated cell culture Real-Time quantitative PCR (ICC-qPCR)) and Ethidium monoazide quantitative PCR (EMA-qPCR) to be applied to detect the infectious adenovirus in environmental water, and further to evaluate the infection risk by adenovirus. The results of optimum conditions for ICC-qPCR was appeared that the lowest detection limit for adenovirus was amplified by 2 ~ 3 log PFU / L and the detection range from 3.4 × 10-1 to 3.4 × 105 PFU / ml within 24 hours cells culture. According to the result of the optimum conditions for EMA-qPCR, the optimal concentration of EMA was 30μg/ml, and the optimal illumination time was 20 minutes. Besides, it was estimated the ratio of dead viruses to viable viruses was no more than 103, and the detection range from 3.37 to 10.7 log copies / mL. The optimum conditions for both quantative method were also applied to detect the infectious adenovirus from environmental water, including sewage water and tap water. The quantities of infectious adenovirus particle detected by EMA-qPCR method were decreased by increasing wastewater treatment procedure. There were no clear difference between three sewage treatment water for the infectious adenovirus numbers detected by ICC-qPCR method. In the light of the result of tap water, there were only 50 % detection rate by EMA-qPCR method. Therefore, the EMA-qPCR method was lower than other methods to be applied to detect infectious adenovirus in environmental water , which suggests that EMA-qPCR can not be applied to be used in the water after disinfection, and might be underestimated the adenovirus infection risk. In 2009 , the amount of sewage treatment plants adenovirus vulnerable to physical and chemical changes in factors (such as water temperature, ammonia nitrogen, dissolved oxygen, residual chlorine, BOD and COD) of the impact, but also with the indicator microorganism E. coli and total coliform Relevant bacteria. Beside, the removal efficiency of total and infectious adenovirus also increased to 74.05% and> 99%, respectively and the high concentration of the total amount of adenovirus was detected in the winter.
    Advisor Committee
  • Chien-Hsien CHen - advisor
  • none - co-chair
  • none - co-chair
  • Files indicate not accessible
    Date of Defense 2011-01-25 Date of Submission 2011-02-15


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