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URN etd-0218113-122506 Statistics This thesis had been viewed 891 times. Download 0 times. Author Tsang-Yi Hsu Author's Email Address No Public. Department Bioengineering Year 2012 Semester 1 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 120 Title The effect of cultural factors on the production of flavonoids in antimitotic agents treated Belamcanda chinensis(L.)DC adventitious roots Keyword flavonoids Belamcanda chinensis(L.)DC. adventitious roots adventitious roots Belamcanda chinensis(L.)DC. flavonoids Abstract Five lines of Belamcanda chinensis(L.)DC. adventitious roots, which respectively named as C05, O05, 2C1, 3C1 and T05 have been established by antimitotic agents treatment. The aim of the thesis is to culture these five lines of adventitious roots under different cultural factors: different root length and wounding, inocula and subculture periods in order to obtain the adventitious root line with high growth ratio and flavonoids production. Among different culture test, T05 line ( 3-4 cm root length and 0.06 g/10 ml inoculation) expressed the highest growth ratio (27.81±1.4) and total flavonoids content was up to 86.48±5.97 eq. iridin mg/g DW and the total of isoflavonoids content was 45.83±1.22 mg/g DW after four weeks culture. In bioreactor culture, T05 line has a highest growth ratio (13.49±0.56) and total flavonoids content (48.31±1.48 eq. iridin mg/g DW), when culture in modified MS medium with 2% sucrose and 1 g/100 ml inoculums. The content of total fiavonoids and isofiavonoids of T05 line was increased when cultured in MS medium added with methyl jasmonate. The growth ratio and total flavonoids content of T05 line and wild type adventitious roots were compared. The growth ratio of T05 line is up to ten folds higher than the wild type in 2L bioreactor culture. The total flavonoids content of T05 line is two folds higher than rhizome growing in the field. Advisor Committee Chin-Wen Ho - advisor
Files Date of Defense 2013-01-28 Date of Submission 2013-02-18