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URN etd-0222110-163404 Statistics This thesis had been viewed 3148 times. Download 2514 times. Author Sung-En Chen Author's Email Address No Public. Department Bioengineering Year 2009 Semester 1 Degree Master Type of Document Master's Thesis Language Chinese&English Page Count 42 Title Investigation of the Problems Associated with Capillary DNA Sequencing Keyword DNA-sequencing Capillary-electrophoresis Capillary-electrophoresis DNA-sequencing Abstract All creatures on earth are constructed with cells. Cells contain genetic materials which are made of DNA. All living things are characterized with the proteins expressed by codes on their DNA. DNA is a polymer of nucleotides. Each nucleotide is composed of pentose, phosphate, purine or pyrimidine. The genetic message is governed by the sequence of purine and pyrimidine. Sanger method which is specified by selective termination of DNA-polymerase, is a primary process to read the codes on DNA. The modern DNA-sequencing technique, an important tool in molecular biology, is a combination of Sanger method and capillary electrophoresis. Success in DNA-sequencing depends on the properties of sample and primer, and the skill of experimental operator. The factors affecting the results of DNA-sequencing are investigated in this work. Advisor Committee Dey-Chyi Sheu - advisor
Files Date of Defense 2010-01-19 Date of Submission 2010-02-22