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Title page for etd-0222110-163404


URN etd-0222110-163404 Statistics This thesis had been viewed 3210 times. Download 2514 times.
Author Sung-En Chen
Author's Email Address No Public.
Department Bioengineering
Year 2009 Semester 1
Degree Master Type of Document Master's Thesis
Language Chinese&English Page Count 42
Title Investigation of the Problems Associated with Capillary DNA Sequencing
Keyword
  • DNA-sequencing
  • Capillary-electrophoresis
  • Capillary-electrophoresis
  • DNA-sequencing
  • Abstract All creatures on earth are constructed with cells. Cells contain genetic materials which are made of DNA. All living things are characterized with the proteins expressed by codes on their DNA. DNA is a polymer of nucleotides. Each nucleotide is composed of pentose, phosphate, purine or pyrimidine. The genetic message is governed by the sequence of purine and pyrimidine. Sanger method which is specified by selective termination of DNA-polymerase, is a primary process to read the codes on DNA. The modern DNA-sequencing technique, an important tool in molecular biology, is a combination of Sanger method and capillary electrophoresis. Success in DNA-sequencing depends on the properties of sample and primer, and the skill of experimental operator. The factors affecting the results of DNA-sequencing are investigated in this work.
    Advisor Committee
  • Dey-Chyi Sheu - advisor
  • Files indicate access worldwide
    Date of Defense 2010-01-19 Date of Submission 2010-02-22


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