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URN etd-0224104-144806 Statistics This thesis had been viewed 2937 times. Download 1761 times. Author Hsin-Yu Lai Author's Email Address No Public. Department Bioengineering Year 2003 Semester 1 Degree Master Type of Document Master's Thesis Language English Page Count 64 Title Establishment of an authentic hepatitis C virus infection and replication system Keyword HCV Cell line Cell line HCV Abstract Hepatitis C Virus (HCV) is a member of the family Flaviviridae. It is the major causative agent of parenterally transmitted non-A non-B hepatitis. HCV infection may lead to chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. Previously, we have identified a hepatic factor capable of supporting HCV replication in an otherwise nonpermissive cell line, 293 EBNA cells. This factor exhibits homology to a group of protein derived from various evolutionarily distant species, including Oryza sativa submergence-induced protein 2A, temporarily named“submergence induced protein-like factor ”(Sip-L). We have utilized 293EBNA-Sip-L system and a newly developed human liver slice method to test for anti-viral activities of several candidate agents. Sodium stibogluconate, previously used to treat leshimaniasis, was found to be capable of suppressing HCV replication. Subsequently, we have transfected Hepa1-6 cells (mouse hepatoma cells) with CD81 and Sip-L cDNA genes. The transfected cells became permissive for HCV infection and replication. Advisor Committee Chau-ting Yeh - advisor
I-ching Kuan - advisor
Ming-Tse Lin - co-chair
Files Date of Defense 2004-01-16 Date of Submission 2004-02-24