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Title page for etd-0313106-122728


URN etd-0313106-122728 Statistics This thesis had been viewed 2150 times. Download 18 times.
Author Tzu-ling Chen
Author's Email Address linlin1018@pchome.com.tw
Department Bioengineering
Year 2005 Semester 1
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 131
Title Heterologous expression of the Saccharomyces cerevisiae alcohol acetyltransferase I gene in Pichia pastoris for production of
isoamyl acetate
Keyword
  • Saccharomyces cerevisiae
  • Pichia pastoris
  • heterologous expression
  • alcohol acetyltransferase
  • alcohol acetyltransferase
  • heterologous expression
  • Pichia pastoris
  • Saccharomyces cerevisiae
  • Abstract Isoamyl acetate is an important flavor compound in wine and beverage.
    It is formed from isoamyl alcohol and acetyl-CoA catalyzed by alcohol
    acetyltransferase (AATase) in Saccharomyces cerevisiae. In this study, the
    gene ATF1 coded for AATase from S. cerevisiae BCRC 21731 was cloned
    and constructed into a constitutive expression vector pGAPZA. A
    recombinant Pichia pastoris strain was obtained express high level natural
    flavor to produce isoamyl acetate. The optimal culture condition to enhance
    the production of isoamyl acetate by recombinant P. pastoris was also
    investigated.
       The results showed that recombinant strain GS115/GAPZA-ATF1
    produced twice as much isoamyl acetate as GS115/GAPZA (control strain).
    When glucose, other than glycerol or methanol, was used as carbon source,
    recombinant strain produced the highest amount of isoamyl acetate. The
    optimal medium composition for isoamyl acetate production by recombinant
    strain was 2% glucose, 1% yeast extract and 20 mM isoamyl alcohol. The
    highest production of isoamyl acetate, is 0.87 mM, was obtained when the
    recombinant strain GS115/GAPZA-ATF1 was cultivated in the optimal
    medium at 30℃ for 4 days.
    Advisor Committee
  • Shiow-Ling Lee - advisor
  • I-Ching Kuan - co-chair
  • Ruey-Chih Su - co-chair
  • Files indicate in-campus access only
    Date of Defense 2006-01-20 Date of Submission 2006-03-13


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