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URN etd-0313106-122728 Statistics This thesis had been viewed 2217 times. Download 18 times. Author Tzu-ling Chen Author's Email Address email@example.com Department Bioengineering Year 2005 Semester 1 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 131 Title Heterologous expression of the Saccharomyces cerevisiae alcohol acetyltransferase I gene in Pichia pastoris for production of
Keyword Saccharomyces cerevisiae Pichia pastoris heterologous expression alcohol acetyltransferase alcohol acetyltransferase heterologous expression Pichia pastoris Saccharomyces cerevisiae Abstract Isoamyl acetate is an important flavor compound in wine and beverage.
It is formed from isoamyl alcohol and acetyl-CoA catalyzed by alcohol
acetyltransferase (AATase) in Saccharomyces cerevisiae. In this study, the
gene ATF1 coded for AATase from S. cerevisiae BCRC 21731 was cloned
and constructed into a constitutive expression vector pGAPZA. A
recombinant Pichia pastoris strain was obtained express high level natural
flavor to produce isoamyl acetate. The optimal culture condition to enhance
the production of isoamyl acetate by recombinant P. pastoris was also
The results showed that recombinant strain GS115/GAPZA-ATF1
produced twice as much isoamyl acetate as GS115/GAPZA (control strain).
When glucose, other than glycerol or methanol, was used as carbon source,
recombinant strain produced the highest amount of isoamyl acetate. The
optimal medium composition for isoamyl acetate production by recombinant
strain was 2% glucose, 1% yeast extract and 20 mM isoamyl alcohol. The
highest production of isoamyl acetate, is 0.87 mM, was obtained when the
recombinant strain GS115/GAPZA-ATF1 was cultivated in the optimal
medium at 30℃ for 4 days.
Advisor Committee Shiow-Ling Lee - advisor
I-Ching Kuan - co-chair
Ruey-Chih Su - co-chair
Files Date of Defense 2006-01-20 Date of Submission 2006-03-13