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Title page for etd-0405104-163336


URN etd-0405104-163336 Statistics This thesis had been viewed 1923 times. Download 8 times.
Author Shu-Ching Weng
Author's Email Address No Public.
Department Bioengineering
Year 2003 Semester 2
Degree Master Type of Document Master's Thesis
Language English Page Count 108
Title PRODUCTION OF SOLAMARGINE FROM PULP CELL CULTURE OF SOLANUM INCANUM
Keyword
  • Solanum incanum
  • solamargine
  • pulp
  • peel
  • cell suspension culture
  • callus
  • callus
  • cell suspension culture
  • peel
  • pulp
  • solamargine
  • Solanum incanum
  • Abstract The fastest growing calli were induced from fruits pulp (1~2 cm ) explant of Solanum incanum on MS basal medium contained with NAA plus BA. In cell suspension culture, cell fresh weight achieved 3.88 g/ml and solamargine accumulation 150.04 mg/g.DW when cell cultured in MS+0.2 mg/l NAA+2.25 mg/l BA after 18 days culture. Addition jasmonic acid (2 mg/l, 4 mg/l, 6 mg/l) to liquid suspension medium significantly promote solamargine accumulation in cell and jasmonic acid at 2 mg/l showed the best cell multiplication (biomass up to 11-fold) and solamargine accumulation 206.09 mg/g.DW.
    Cell was scaled up by flask. In a two stage culture obtained the best cell multiplication and solamargine accumulation. After 18 days, fresh weight of cell was increased from 13.01 g to 36.01 g and the accumulation of solamargine in cell was 306.21 mg/g.DW .
    Advisor Committee
  • Chin-Wen Ho - advisor
  • Kow-Jen Duan - co-chair
  • Wei-Chin Chang - co-chair
  • Files indicate in-campus access only
    Date of Defense 2003-12-18 Date of Submission 2004-04-05


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