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URN etd-0405104-163336 Statistics This thesis had been viewed 1923 times. Download 8 times. Author Shu-Ching Weng Author's Email Address No Public. Department Bioengineering Year 2003 Semester 2 Degree Master Type of Document Master's Thesis Language English Page Count 108 Title PRODUCTION OF SOLAMARGINE FROM PULP CELL CULTURE OF SOLANUM INCANUM Keyword Solanum incanum solamargine pulp peel cell suspension culture callus callus cell suspension culture peel pulp solamargine Solanum incanum Abstract The fastest growing calli were induced from fruits pulp (1~2 cm ) explant of Solanum incanum on MS basal medium contained with NAA plus BA. In cell suspension culture, cell fresh weight achieved 3.88 g/ml and solamargine accumulation 150.04 mg/g.DW when cell cultured in MS＋0.2 mg/l NAA＋2.25 mg/l BA after 18 days culture. Addition jasmonic acid (2 mg/l, 4 mg/l, 6 mg/l) to liquid suspension medium significantly promote solamargine accumulation in cell and jasmonic acid at 2 mg/l showed the best cell multiplication (biomass up to 11-fold) and solamargine accumulation 206.09 mg/g.DW.
Cell was scaled up by flask. In a two stage culture obtained the best cell multiplication and solamargine accumulation. After 18 days, fresh weight of cell was increased from 13.01 g to 36.01 g and the accumulation of solamargine in cell was 306.21 mg/g.DW .
Advisor Committee Chin-Wen Ho - advisor
Kow-Jen Duan - co-chair
Wei-Chin Chang - co-chair
Files Date of Defense 2003-12-18 Date of Submission 2004-04-05