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Title page for etd-0616111-165521


URN etd-0616111-165521 Statistics This thesis had been viewed 1964 times. Download 9 times.
Author Ching-Wen Hsu
Author's Email Address No Public.
Department Bioengineering
Year 2010 Semester 2
Degree Master Type of Document Master's Thesis
Language English Page Count 101
Title Effect of Lactic Acid Bacteria-fermented Sake Lees on Activating CYP2E1 Activity and Scavenging ROS in HepG2 Cell
Keyword
  • CYP2E1
  • lactic acid bacteria
  • sake lees
  • antioxidant
  • antioxidant
  • sake lees
  • lactic acid bacteria
  • CYP2E1
  • Abstract In the hepatocyte, ethanol is metabolized to acetaldehyde by alcohol dehydrogenase (ADH) or cytochrome P4502E1 (CYP2E1). Then, acetaldehyde is metabolized to acetic acid by aldehyde dehydrogenase (ALDH). CYP2E1 plays a significant role in regenerating reactive oxygen species (ROS) as well as oxidative stress. Chronic alcoholism induces the pressure of alcohol, and it can increase the level of CYP2E1, which in turn results in the generation of more ROS, exacerbating the potential for cell and tissue damage. In this study, lactic acid bacteria-fermented sake lees were used to test their antioxidant ability and affects on CYP2E1, ADH and ALDH activity. Sake lees fermented by Lactobacillus casei E7 and Lactobacillus acidophilus V12 have the highest antioxidant abilities. Also, the CYP2E1 activities from these two LAB-fermented sake lees culture filtrates are increased by 2.0 folds and 1.5 folds, respectively. ALDH activities are increased by 1.2 folds and 1.1 folds, respectively. However, there is no affects on the activity of ADH. Using Sephacryl 100HR to purify protein and run a native-PAGE and SDS-PAGE to check its purity and molecular weight. The molecular weight of purified protein is about 30 kDa and its isoelectric focusing point is close to 5.85. In conclusion,SE7-fermented sake lees culture filtrate not only increase CYP2E1 activity but also have the antioxidant ability. This helps alleviate the cell damages caused by chronic alcoholism.
    Advisor Committee
  • Tsong-Rong Yan - advisor
  • none - co-chair
  • none - co-chair
  • Files indicate in-campus access at 1 years and off-campus not accessible
    Date of Defense 2011-06-01 Date of Submission 2011-07-04


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