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URN etd-0629104-174438 Statistics This thesis had been viewed 2777 times. Download 3783 times. Author Chih-feng Chang Author's Email Address No Public. Department Bioengineering Year 2003 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 134 Title Optimization of the medium components for Tricholoma matsutake submerged culture Keyword Tricholoma matsutake submerged culture response surface methodology mushroom fungi endoplysaccharide endoplysaccharide fungi mushroom response surface methodology submerged culture Tricholoma matsutake Abstract Tricholoma matsutake, also named matsutake, is a precious edible fungus which shows many pharmacological activities including anti-tumor effect and immunology enhancement. In this study, we investigated the effects of adding different carbon, amino acid, ammonium nitrogen and organic nitrogen (peptone and yeast extract) in T. matsutake BCRC 36614 submerged cultures on the growth and endopolysaccharide formation. Finally, we used response surface methodology to find the optimal concentration of each medium component. Our results showed that glucose, maltose, mannose, lactose and malt extract promoted not only the growth of T. matsutake BCRC 36614, but also the formation of endopolysaccharide. Among them, T. matsutake BCRC 36614 cultured in 2 % glucose-added basal medium after 40 days could result in maximal cell dry weight (8.4 g/l), endopolysaccharide content (3.6 mg/ 100mg CDW), and maximal endopolysaccharide production (30.1 mg/ 100 ml). Addition of L-arginine or L-glutamic acid promoted not only the growth of T. matsutake BCRC 36614, but also the formation of endopolysaccharide. However, two ammonium nitrogen, ammonium tartrate and ammonium nitrate, showed suppressive effects. The best growth of T. matsutake BCRC 36614 was obtained in 0.2 % L-arginine-added medium. In the 40-day culture, we obtained maximal cell dry weight (9.9 g/l) which was 1.2 times compared with the control group, maximal endopolysaccharide content (7.4 mg/ 100mg CDW) which was 2 times compared with its control group, and maximal production (73.4 mg/ 100 ml). Addition of peptone or yeast extract could significantly affect the growth and endopolysaccharide formation of T. matsutake BCRC 36614. The optimal concentrations of peptone and yeast extract were both 0.2 %. Using response surface methodology, we found the optimal medium components formula: 2.25 % glucose, 0.2 % peptone, 0.22 % yeast extract, 0.2 % L-arginine, 0.05 % KH2PO4, and 0.05 % MgSO4•7H2O. Cultured in the optimal medium, T. matsutake BCRC 36614 produced maximal endopolysaccharide content (7.3 mg/100 mg CDW). Advisor Committee none - advisor
none - co-chair
none - co-chair
Files Date of Defense 2004-06-28 Date of Submission 2004-06-29