||The objective of the study is to explore day to day applications of pineapple eco-enzyme. The investigators first analyzed the strains of pineapple eco-enzyme, and then a questionnaire survey was conducted among 34 randomly sampled subjects. The questionnaire used in the survey was developed by the investigators. In addition to the questionnaire survey, these 34 subjects also participated in an experiment (one month duration) in which they were asked to use the pineapple eco-enzyme detergent for dish washing, cleaning laundry, and floor cleaning, and the usage condition was analyzed. Lastly, the investigators compared between the pineapple eco-enzyme liquid fertilizer (the experimental group) and water (the control group) in terms of their effects on plants. Experimental and statistical analysis results showed that pineapple eco-enzyme uses lactose to produce lactate bacteria. When using the pineapple eco-enzyme detergent for dish washing, the level of cleanliness was found to be significantly associated with enzyme concentration; the cleaning power of higher concentration (1:2) was better than of lower concentration (1:10). As for reducing dry hands and odor acceptance, no significant concentration effect was found. In terms of applying the pineapple eco-enzyme detergent on cleaning laundry and floor cleaning, the subjects revealed a lack of significant concentration effect on the cleaning power, reducing dry hands, or odor acceptance. In addition, among subjects supporting pineapple eco-enzyme usage and actively asking for more eco-enzyme related information, no significant concentration, gender or education effect was found. Lastly, when the pineapple eco-enzyme liquid fertilizer was used in planting, it was found that plant growth was not suppressed by the eco-enzyme liquid fertilizer. On the contrary, potted plants receiving either the 100-fold or 500-fold dilution were found to grow better than potted plants in the control group (receiving water only). Moreover, the eco-enzyme liquid fertilizer, compared to water, also showed a better plant disease control effect.