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Title page for etd-0707104-011506


URN etd-0707104-011506 Statistics This thesis had been viewed 1406 times. Download 27 times.
Author Wan-Chen Chan
Author's Email Address No Public.
Department Materials Engineering
Year 2003 Semester 2
Degree Master Type of Document Master's Thesis
Language English Page Count 117
Title The Study of Immobilization of DNA on Plasma and Graft Polymerization Treated QCM Surface for Food Pathogens Detective
Keyword
  • surface graft polymerization
  • QCM
  • Plasma polymerization
  • Food pathogens detection
  • DNA immobilization
  • DNA immobilization
  • Food pathogens detection
  • Plasma polymerization
  • QCM
  • surface graft polymerization
  • Abstract Quartz crystal microbalances (QCMs) are transducers for chemical and biochemical sensing in general. The oscillation frequency of QCM will be affected when the adsorbed mass on the surface changed, so in view of this characteristic, it can be used widely to all kinds of mass sensor applications. If immobilized the NH2-biomolecule on QCM surface. Such as single strand DNA, then from the frequency shift measured can detect DNA hybridization reaction. In this study, the HMDSZ plasma is utilized to deposit organic silicon thin film on the surface of QCM in order to make an interface layer between the inorganic material and organic molecule. Consequently, the surface UV-light grafting polymerization of AAm can provide useful functional sites for binding enzyme or the DNA fragment of bacillus. Hence, GA as cross-linking reagent to chemical bonding NH2-oligonucleotide probes on the surface of QCM. Experimental results indicated that combination of plasma deposition (HMDSZ 50 mtorr、1min、20W) and graft polymerization (AAm 10 wt%、1000W、20min); the biomolecul with –NH2 groups (such as DNA probe) can be immobilization effectively on QCM surface. And it has good detection sensitivity for the pathogenic gene eaeA of Escherichia coli O157:H7 than other control experiments; meanwhile the fabricated DNA-QCM sensor could differentiate from the other treated bacteria.
    Advisor Committee
  • Ko-Shao Chen - advisor
  • Hong-Ru Lin - co-chair
  • Tsong-Rong Yan - co-chair
  • Files indicate in-campus access only
    Date of Defense 2004-06-17 Date of Submission 2004-07-07


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