||Chitosan sponge, which is a biodegradable and antibacterial polymer immobilized onto the surface of PNIPAAm gel/Poly (ethylene terephthalate) (PET) non-woven composites surface for application in wound dressing was studied. Poly (ethylene terephthalate) non-woven has extensively using due to its porosity, allowing ventilation, high surface area and excellent mechanical properties. However, the hydrophobic surface of PET non-woven had limited on its application, so, we tried to use the plasma-activation treatment and subsequently UV-light graft polymerization of NIPAAm gel to improving the compatibility of PET non-woven with PNIPAAm gel. The results indicated that the wet ability was improved after plasma treatment and photo-induced graft. Graft monomer was N-isopropylacrylamide (NIPAAm), which has specific thermo-sensitive property. The substrate of a easy strip surface was prepared by introduction of NIPAAm monomer onto PET non-woven, these complexes were characterized by prepared chitosan porous scaffold, finally, chitosan was immobilized onto PNIPAAm gel/PET non-woven composites surface using freeze-dried treatment and the cross-linking reagent, Na5P3O10. The designed products were analyzed by FT-IR and scanning electron microscopy (SEM). Porous structure was shown in SEM pictures. Chitosan sponge in vivo likely status for 30 days and chitosan sponge remain rate was 76%. So chitosan sponge was bio-degradation material, and degradation rate wasn’t too fast. Antibacterial activities will be executed. For cell culture, cells can grow in chitosan sponge, and mouse embryonic stem cells grew in chitosan sponge that treatment by Ar plasma was not well. Then, mouse embryonic stem cells grew in chitosan sponge and chitosan sponge that with 10μl type I collage and tissue culture plate were similar. In animal test, chitosan tri-layer wound dressing make mice wound healed after 12 days, and no inflammation status. And it can be expected to applicative in wound dressing.