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URN etd-0718106-143449 Statistics This thesis had been viewed 5862 times. Download 2801 times. Author Kuo-Wei Chen Author's Email Address email@example.com Department Bioengineering Year 2005 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 107 Title Detection of the Radical-Scavenging Abilities of six Chinese herbal medicines by Probe-Based Ultraweak Chemiluminescence Technique Keyword Ultraweak Chemiluminescence Ultraweak Chemiluminescence Abstract Our propensity to relate everything back to the natural world has globally initiated an emerging trend to once again focus on nature and its impact on Chinese herbal medicine. The art and science of today’s Chinese herbal medicine is the attribution from thousands of years of ancestral knowledge and practices. In addition, the Chinese apothecary treasures an array of the oldest and most efficacious remedies to combat and treat aging. To name a few, Ginseng and St. John’s wort both are the epitomes of the Chinese herbal medicine, which have slowly but successfully gained their popularity into the global mainstream of today’s pharmaceutical market.
Thus, we will select six legendarily prominent Chinese herbal medicines to be our main research objectives. Our intent for this study is to understand its anti-oxidation chemistry and to find a direct correlation between Chinese herbal medicine and its effect as an antioxidant agent. We used a novel probe-based ultraweak chemiluminescence (uwCL) technique capable of detecting a panel of three oxygen-derived free radicals (ODFRs) including superoxide anion (O2•-), hydroxyl radical (•OH) and peroxyl radical (ROO•).
Our approach is to use this technique to assess the radical-scavenging ability (RSA) of the following six popular Chinese herbal medicine: 1.Mesona chinensis Benth, 2.Licorice, 3. Ginkgo leaf, 4.Olive leaf, 5.Green tea, 6.Cassia bark. By comparing the difference of the IC50 values of each material, we were able to get the sequence of the six Chinese herbal medicine based on their RSA. For 1) O2-.scavenging ability, the order of inhibitory efficiency were: 5.(1.37 μg)> 1.(1.78 μg)> 6.(3.15 μg)> 4.(16.69 μg)> 2.(18.45 μg)> 3.(22.82 μg), respectively; for 2) •OH -scavenging ability, the order of inhibitory efficiency were: 5.(3.36 μg)> 1.(4.15 μg)> 6.(23.82 μg)> 4.(28.63 μg)> 2.(43.53 μg)> 3.(46.71 μg), respectively; for 3) ROO• -scavenging ability were 1.(0.18 TEU)> 5.(0.14 TEU)> 6.(0.04 TEU)> 4.(0.03 TEU)> 3.(0.023 TEU)> 2.(0.020 TEU), respectively. The test results help us know how to choose healthcare products in our daily lives, specifically how to intake the recommended dosage of antioxidants.
Key words—Mesona chinensis Benth, Licorice, Ginkgo leaf, Olive leaf, Green tea, Cassia bark, Anti-oxidation, Free radical, Ultraweak Chemiluminescence (uwCL), TEU (Trolox-Equivalent Unit)
Advisor Committee Prof. Kow-Jen Duan - advisor
Chi-Tsai Lin - co-chair
Prof. Jin-Wen Ho - co-chair
Files Date of Defense 2006-07-04 Date of Submission 2006-07-18