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URN etd-0720106-142655 Statistics This thesis had been viewed 6019 times. Download 2043 times. Author Hsing-Jung Ching Author's Email Address email@example.com Department Bioengineering Year 2005 Semester 2 Degree Master Type of Document Master's Thesis Language English Page Count 61 Title Influence of Ammoniacal Concentration on L-Lactic Acid Production by Culture of Rhizopus oryzae Keyword Rhizopus oryzae lactic acid production fungal morphology bioreactor bioreactor fungal morphology lactic acid production Rhizopus oryzae Abstract A fermentation process for enhanced production of L(+)-lactic acid was developed. Rhizopus oryzae cultured with glucose as carbon source exhibited floc morphology in a stirred tank bioreactor. The effects of fermentation pH as well as the initial concentration of ammonium sulfate added to the culture medium on LA production were investigated. Optimal production of LA was achieved in the condition of pH 4.3 – 4.4 with initial concentration of AS at 4 g/L after 36 h of fermentation. The final concentration of LA was 104 g/L with a yield of 87%. Furthermore, the average concentration and the yield of lactic acid in the following four cycles of the 28-h repeated batch fermentation were up to 109 g/L and 91%, respectively, using 3 g/L of ammonium sulfate as nitrogen source. Advisor Committee Prof. Dey-Chyi Sheu - advisor
none - co-chair
none - co-chair
Files Date of Defense 2006-07-20 Date of Submission 2006-07-20