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URN etd-0724113-154501 Statistics This thesis had been viewed 1736 times. Download 620 times. Author chia-hui chang Author's Email Address No Public. Department Bioengineering Year 2012 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 143 Title Preparation of Poly-hydroxy-butyrate-co-hydroxyalerate (PHBV) / Poly-ε-caprolactone (PCL) Membrane by Electrospinning for Tissue Engineering Keyword Tissue engineering plasma modification electrospinning cell compatibility cell compatibility electrospinning plasma modification Tissue engineering Abstract Abstract
In recent years, development of tissue engineering becomes an important issue.Cartilage defects caused by trauma or disease are difficult to recover because articular cartilage can’t regenerate by itself . Although allogeneic cartilage has been used , it may lead to rejection, cell death or the loss of function. Therefore, cartilage tissue with synthetic scaffold and autologous cells becomes important research directions.
This study used electrospinning method to prepare scaffold for cartilage engineering. Biodegradable poly-hydroxybutyrate-co-hydroxyvalerate (PHBV) and polymer material poly-ε-caprolactone (PCL) was chosen as the raw materials. We adjust PHBV content to improve the mechanical properties and degradation rates of the PCL. Polymers blended with different proportion of PHBV/PCL (100/0, 75/25, 50/50, 25/75, 0/100) to form the films. The films were characterized with contact angle measurement, calorimetry (DSC) , Lloyd test machine,and scanning electro microscopy (SEM). PHBV/PCL(50/50) Fiber have the best physical properties, then can be used in plasma modification .To enhance the cell growth rate, we further treated the electropsun fiber with different plasma surface (Oxygen, Hexamethyldisilazane, Oxygen + Hexamethyldisilazane) to modify hydrophilicity of the surface .The results of this study showed that plasma surface modification had improved the cell growth.The hydrophobic fiber had higher growth rate then the hydrophilic fiber.
Advisor Committee Chung-Yih Wang - advisor
Chien-Chung Chen - co-chair
Ko-Shao Chen - co-chair
Files Date of Defense 2013-07-03 Date of Submission 2013-07-25