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URN etd-0725115-082127 Statistics This thesis had been viewed 1038 times. Download 84 times. Author Kuo-Fon Ma Author's Email Address No Public. Department Bioengineering Year 2014 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 82 Title Evaluation of Various Origins and Extracting Methods of Star-anise for Anti-Pseudomonas aeruginosa Keyword Spirit extract antibacterial activity star anise Pseudomonas aeruginosa water extract ethanol extract supercritical carbon dioxide supercritical carbon dioxide ethanol extract water extract Pseudomonas aeruginosa star anise antibacterial activity Spirit extract Abstract Pseudomonas aeruginosa was a major pathogen causing nosocomial infection, because it could survive in moist environments and was so adaptable that often causes a number of vehicle incidents of nosocomial infection group. It has been reported by Taipei Veterans Hospital, Taoyuan branch that from 2011 to 2014 among various bacteria causing nosocomial infection, Acinetobacter baumannii, E. coli and P. aeruginosa were the first 3 ranked (by 29, 28 and 18%). Particularly in the medical community infections by multi-drug resistant P. aeruginosa are hard to treat.
Star anise is obtained from the star-shaped pericarp of Illicium verum. Star anise is a spice often used in cooking. Beside, several ingredients in star anise have antibacterial activities in nature. The extract of star anise is to be a potent antibacterial agent and can be used in food preservation, cosmetics and healthcares.
According to this viewpoint, in this work, star anises obtained from Taiwan, China (Guangxi) and Vietnam were extracted by four different methods and the inhibitory effects of these extracts against P. aeruginosa were compared. The results were summarized as follows: (1) supercritical CO2 extract, Spirit extract and ethanol extract effectively inhibit P. aeruginosa, whereas aqueous extract did not. (2) by supercritical CO2 extraction, anise of Vietnam origin gave the highest antibacterial effect. The antibacterial effects of star anises from different areas were ranked in the follow sequence: Vietnam > China > Taiwan. (3) by Spirit extraction, anise of China origin gave the highest antibacterial effect. The antibacterial effects of star anises from different areas were ranked in the follow sequence: China > Vietnam > Taiwan. (4) by ethanol extraction, anise of Taiwan origin gave the highest antibacterial effect. The antibacterial effects of star anises from different areas were ranked in the follow sequence: Taiwan ≧Vietnam > China.
Advisor Committee Dey-Chyi Sheu - advisor
C-Will Chen - co-chair
Chi-Tsai Lin - co-chair
Files Date of Defense 2015-07-23 Date of Submission 2015-07-27