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Title page for etd-0727109-152004


URN etd-0727109-152004 Statistics This thesis had been viewed 1755 times. Download 11 times.
Author An-Ting Cheng
Author's Email Address No Public.
Department Bioengineering
Year 2008 Semester 2
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 93
Title Interaction of LL-37 with Model Membrane Systems and Preparing Lipid Based Biosensor for the Detection of LL-37
Keyword
  • LL-37
  • antimicrobial peptide
  • biosensor
  • biosensor
  • antimicrobial peptide
  • LL-37
  • Abstract It’s well known that bacteria have ability to develop antibiotic-resistance in short time after treatment. To find therapeutic agents with different mode of action than conventional antibiotics is a necessity. To meet the above requirement, antimicrobial peptides (AMPs) represent a good alternative for infective antibiotics. AMP LL-37 with positive charges belongs to an immune system that has a capacity to distinguish membrane from different lipids in human. This special property makes LL-37 destroy the negative charged bacteria membranes. Therefore the above mechanism is effective to drug resistance bacteria. Normally bacteria and LL-37 will not exist in urine, except for those patients who suffer urinary tract infection (UTI). Hopefully, a screening biosensor can be made by using the above character.
    DSC (Differential Scanning Calorimeter) was used to measure interaction between
    LL-37 and liposome that plays a biomembrane role. The stability of the complex can be affected by adding interference salt and urea. In the meantime, the change of liposome structure can be observed by a fluorescence probe on it. Finally, the biosensor was made by immobilizing liposomes on silane coated surface. Testing was made by mixing the sample containing LL-37 on the biosensor plate. The content of LL-37 can be detected and determined by impedance and phase angle variations.
    Advisor Committee
  • Chung-Yih Wang - advisor
  • none - co-chair
  • none - co-chair
  • Files indicate in-campus access at 2 years and off-campus not accessible
    Date of Defense 2009-06-26 Date of Submission 2009-07-29


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