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Title page for etd-0730108-111712


URN etd-0730108-111712 Statistics This thesis had been viewed 2783 times. Download 1401 times.
Author Fang-Yi Chen
Author's Email Address No Public.
Department Bioengineering
Year 2007 Semester 2
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 84
Title Flavonoids production by adventitious root culture of Belamcanda chinensis(L.)DC.
Keyword
  • Flavonoids
  • Belamcanda chinensis(L.)DC.
  • Belamcanda chinensis(L.)DC.
  • Flavonoids
  • Abstract The growth ratio of callus of Belamcanda chinensis reached to 9.3 times when cultured in MS liquid medium without growth regulators for 4 weeks. Callus regenerated into adventitious roots gradually during cultural period. Total flavonoid of methanolic extract in adventitious roots was 12.99±1.55 eq. iridin % DW. Five kinds of flavonoids (tectoridin , iridin , tectorigenin , isorhamnetin and irigenin ) in methanolic extract were analyzed by HPLC. Mature adventitious roots (> 3cm) were cultured in MS liquid medium supplemented with L-phenylalanine or yeast extract produced high isoflavonoid(3.8~4.1 % DW). For scale up, adventitious roots were cultured in 1L bubble column bioreactor in 500 ml MS liquid medium for 4 weeks resulted 3.6-fold increase in biomass. The methanolic extracts in adventitious roots cultured in bubble column bioreactor exhibited 1.3-fold in total flavonoid and 1.2-fold in total isoflavonoid, compared to 3-year-old medicinal rhizome. Tectoridin was major isoflavonoid in medicinal rhizome but iridin accumulated in large amount in adventitious root by in vitro culture.
    Advisor Committee
  • Chin-Wen Ho - advisor
  • Kow-Jen Duan - co-chair
  • Mei-Hsien Lee - co-chair
  • Files indicate in-campus access at 3 years and off-campus access at 3 years
    Date of Defense 2008-06-18 Date of Submission 2008-07-30


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