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URN etd-0730108-111712 Statistics This thesis had been viewed 2828 times. Download 1401 times. Author Fang-Yi Chen Author's Email Address No Public. Department Bioengineering Year 2007 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 84 Title Flavonoids production by adventitious root culture of Belamcanda chinensis（L.）DC. Keyword Flavonoids Belamcanda chinensis（L.）DC. Belamcanda chinensis（L.）DC. Flavonoids Abstract The growth ratio of callus of Belamcanda chinensis reached to 9.3 times when cultured in MS liquid medium without growth regulators for 4 weeks. Callus regenerated into adventitious roots gradually during cultural period. Total flavonoid of methanolic extract in adventitious roots was 12.99±1.55 eq. iridin % DW. Five kinds of flavonoids (tectoridin , iridin , tectorigenin , isorhamnetin and irigenin ) in methanolic extract were analyzed by HPLC. Mature adventitious roots (> 3cm) were cultured in MS liquid medium supplemented with L-phenylalanine or yeast extract produced high isoflavonoid（3.8~4.1 % DW）. For scale up, adventitious roots were cultured in 1L bubble column bioreactor in 500 ml MS liquid medium for 4 weeks resulted 3.6-fold increase in biomass. The methanolic extracts in adventitious roots cultured in bubble column bioreactor exhibited 1.3-fold in total flavonoid and 1.2-fold in total isoflavonoid, compared to 3-year-old medicinal rhizome. Tectoridin was major isoflavonoid in medicinal rhizome but iridin accumulated in large amount in adventitious root by in vitro culture. Advisor Committee Chin-Wen Ho - advisor
Kow-Jen Duan - co-chair
Mei-Hsien Lee - co-chair
Files Date of Defense 2008-06-18 Date of Submission 2008-07-30