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URN etd-0730112-125333 Statistics This thesis had been viewed 1340 times. Download 275 times. Author Ming-Ying HSIEH Author's Email Address No Public. Department Materials Engineering Year 2011 Semester 2 Degree Master Type of Document Master's Thesis Language Chinese&English Page Count 131 Title Surface Modification of PVDF Film to Improve Biomolecule Immobilization and Application in Biosensors Keyword surficial modificat PVDF immuno-sensor plasma plasma immuno-sensor PVDF surficial modificat Abstract Polyvinylidene fluoride (PVDF) has excellent adhesive properties against proteins, high mechanical strength and excellent chemical resistance, it is ideal for a variety of stain and used in the immunoassay. In this study, PVDF were used as matrix of immunosensor to immobilize polyclonal antibody. At first, oxygen plasma was used to activate PVDF’s surface, and then polyethyleneimine (PEI) were coated on activated PVDF surface. At last, immobilized albumin or polycolonal antibody IgY on the PEI-coated PVDF surface with orientation. In the surface modification processing, Quartz crystal microbalance (QCM) were used as a rapid detection method, and PVDF/NMP solution were dropped uniformly onto the surface of QCM sensor. Immobilized biomolecules were performed by QCM in weight change, by AFM for observation of QCM sensor surface roughness. The obtained results plays that the water contact angle of PVDF were decreased from 80.3? to 56.8? after oxygen plasma treatment, and the similar results was also found in the further reaction steps. By the AFM observations, roughness of QCM sensor surface before/after oxygen plasma treatment is 282.6 and 309.2 nm, respectively. In final, the roughness of QCM came to 239.0 nm after PVDF coating. The sensors surface be smoothing by polymeric surficial modification were found. By the FT-IR analysis of PVDF, characteristic absorption peak of hydroperoxide groups at 890~830 cm-1 were found after oxygen plasma treatment, the hydroperoxide achieve further reaction with amino groups in PEI via a covalent binding. The reaction could be used to aid surficial modification to bind various polyclonal or monoclonal antibody as a biosensor. We hope the PVDF membranes with oxygen plasma activation treatment could be apply to prepare a biosensor for in-vitro diagnosis in near future. Advisor Committee Ko-Shao CHEN - advisor
Feng-Huei LIN - co-chair
How TSENG - co-chair
Jen-Ming YANG - co-chair
Mu-Rong YANG - co-chair
Files Date of Defense 2012-07-02 Date of Submission 2012-07-31