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Title page for etd-0801114-151921


URN etd-0801114-151921 Statistics This thesis had been viewed 912 times. Download 2 times.
Author Pei-Jung Hsiao
Author's Email Address No Public.
Department Bioengineering
Year 2013 Semester 2
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 61
Title SILAFFIN R5-MEDIATED BIOMIMETIC SILICIFICATION OF LIPASE FROM CANDIDA RUGOSA
Keyword
  • MNP
  • ubiquitin
  • lipase
  • silaffin R5
  • silaffin R5
  • lipase
  • ubiquitin
  • MNP
  • Abstract In this study, immobilization of Candida rugosa lipase was performed using ubiquitin and silaffin R5 fusion protein, U-R5, as a catalyst and hydrolyzed tetramethoxysilane (TMOS) as the source of silicic acid to generate silica in the presence of magnetic nanoparticle (MNP).To optimize the immobilization, the effects of the amount of U-R5, MNP, lipase and hydrolyzed TMOS added on the encapsulation efficiency and the specific activity of lipase after immobilization were investigated.
    When U-R5, lipase and hydrolyzed TMOS added were increased, the encapsulation efficiencies were decreased but the specific activities of immobilized lipase were increased. Conversely, as the concentrations of MNP added were increased from 1 up to 4 mg/ml, the encapsulation efficiencies and the specific activities both were increased. However, the latter becamelowerat the MNP concentration of 5 mg/ml. The use of 7.5 μM U-R5 or 30 μM PAA to mediate immobilization of lipase gave similar encapsulation efficiencies (~60%), and the specific activitiesof immobilized lipase obtained were also similar to those of free lipase. Nevertheless, the increase in the addition amount of U-R5 and PAA resulted in the contrary effect; Higher PAA concentration displayed better encapsulation efficiency but lower specific activity.
    Advisor Committee
  • I-Ching Kuan - advisor
  • none - co-chair
  • none - co-chair
  • Files indicate in-campus access immediately and off-campus access at 5 years
    Date of Defense 2014-07-23 Date of Submission 2014-08-04


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