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URN etd-0803110-101444 Statistics This thesis had been viewed 2803 times. Download 2807 times. Author Hei-Erh Chang Author's Email Address No Public. Department Bioengineering Year 2009 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 78 Title Quantitative Analysis of Fentanyl in Human Heparin Plasma by LC/ ESI-MS/MS Keyword validation. quantitation plasma HPLC Fentanyl Eelectrospray tandem mass spectrometry tandem mass spectrometry Eelectrospray Fentanyl HPLC plasma quantitation validation. Abstract Fentanyl, a potent analgesic drug, has traditionally been used intravenously in surgical or diagnostic operation. In this study, a simple, sensitive, low sample consumption, and non-derivative method was developed for the quantitative determination of fentanyl in human heparin plasma by liquid chromatography/tandem mass spectrometry (LC/MS/MS). Quantification limit ranging from 20 to 2000 pg/mL was obtained. The analyte response at the lower limit of quantification (LLOQ: 20 pg/mL) was defined as at least 5 times the response compared to blank.
Validation parameters such as specificity/selectivity, limit of quantification (LOQ), linearity, precision, accuracy, recovery, stability and system suitability were evaluated for this diethylether extraction method. No significant interference or matrix effect caused from endogenous compounds was observed at the blank plasma from 6 different sources. The precision (%CV) and accuracy (%RE) of within-run for quality control (QC) of samples ranged from 1.2% to 6.0% and -7.5% to 7.5%, respectively. The precision (%CV) and accuracy (%RE) of between-run for QC of samples ranged from 3.2% to 5.7% and -1.5% to 2.3%, respectively. The precision (%CV) and accuracy (%RE) of LLOQ were 4.9% to 6.8% and -10.5% to 17.0% in within-run, and they were 10.8% and 6.5% in between-run. The method described here is thus eligible for the analysis of biological samples.
Advisor Committee Ming-Tse Lin - advisor
Files Date of Defense 2010-07-08 Date of Submission 2010-08-13