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URN etd-0803115-120343 Statistics This thesis had been viewed 1253 times. Download 1 times. Author Yu-ting Jhou Author's Email Address email@example.com Department Bioengineering Year 2014 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 112 Title Production of Xanthan Gum from Glycerol by Culture of Xanthomonas sp.YT-24 Keyword xanthan gum fermentation glycerol Xanthomonas Xanthomonas glycerol fermentation xanthan gum Abstract Because fossil fuels including coal, petroleum and natural gas are going to run out in the future. Thus, renewable energy sources gained increasing interest, and among these, biodiesel reduced the reliance on petroleum. Recently, as the production of biodiesel increased dramatically, the by-product glycerol increased, too. For the production of 10 kg of biodiesel, 1 kg of glycerol is produced. The glycerol obtained from biodiesel industry is much higher than the traditional demand, led to a substantial decline in the price of glycerol. Therefore, the conversion of glycerol into other substances is a critical problem for the development of biodiesel.
Xanthan gum is commercially available in foods, pharmaceutical formulations, cosmetics, drilling fluids and other industries. But commercially available xanthan gum is relatively expensive due to glucose or sucrose being used as the sole carbon source. Therefore, if glycerol is used as the carbon source for fermentative production of xanthan gum, the production cost of xanthan gum will be significantly reduced. The aim of this work is to produce xanthan gum by fed-batch culture of Xanthomonas sp. YT-24, using glycerol as the substrate. The fermentation conditions are : 28 ± 2℃, 2 vvm, 500 rpm and 120 h. The maximal yield was 17.1 grams (dry weight) per liter of culture broth, with an maximum viscosity of 24.9×103 cP. This is higher than the similar fermentation by X. campestris BCRC 12354. The results showed when xanthan gum fermentation used glycerol as the substrate, Xanthomonas sp. YT-24 was more effective than X. campestris BCRC 12354 .
Advisor Committee Dey-Chyi Sheu - advisor
C. Wil Chen - co-chair
Chi-Tsai Lin - co-chair
Files Date of Defense 2015-07-23 Date of Submission 2015-08-03