||Dengue fever and Dengue hemorrhagic fever are major public health burden in Taiwan and many parts of the world. These diseases are transmitted mainly by Aedes aegypti mosquito in southern part and secondary by Aedes albopictus in whole Taiwan Island. The viral loads of mosquito each day after Aedes aegypti and Aedes albopictus mosquitoes infected with dengue viruses were the topic aim. In this study, we use one-step probe real time RT-PCR method to detect dengue virus serotype 1 and serotype 4 intra-thoracical infected mosquitoes, and the results revealed that virus titers were increased with days and highest on day 8 or 10(DV1 Viral titers 8.1X105,DV4 Viral titers 2.8X106). The Western blot also revealed same pattern that dengue NS1 protein is more prominent on day 8 or 10.
The changes of immune related genes expression in mosquito post dengue virus infection were also our research aim. Of these mosquito innate immune genes, defensin A1, defensin A4 and cecropin B1 can be expressed to against microorganism, such as Gram negative and positive bacteria. Our results revealed these 3 genes were all increased post dengue virus infection, except on day 1. The DVRF1 and DVRF2 gene were reported (Paradkara , et al.2012) for mosquito to confront dengue virus infection; our results revealed that DVRF1 is inhibited, while DVRF2 is induced post dengue virus infection. Vago gene in Culex mosquito is reported (Souza-Neto,J.A.2009) to antagonize West Nile virus, but our result was that it was inhibited in Aedes aegypti mosquito post dengue virus infection. All of above study showed the genes expressions of mosquito innate immune genes ( defensin A1, defensin A4, cecropin B1, and DVRF2 ) were increased post virus infection, while DVRF1 and Vago genes were inhibited post dengue virus infection.
Keywords：one-step probe real time RT-PCR, defensin A1, defensin A4, cecropin B1, DVRF1, DVRF2, Vago