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Title page for etd-0808107-150550


URN etd-0808107-150550 Statistics This thesis had been viewed 4415 times. Download 6 times.
Author Yi-xiu Shih
Author's Email Address No Public.
Department Bioengineering
Year 2006 Semester 2
Degree Master Type of Document Master's Thesis
Language English Page Count 90
Title Production of Chitosan by Submerged Culture of Asperjillus japonicus
Keyword
  • β-fructofuranosidase
  • chitosan
  • Asperjillus japonicus
  • Asperjillus japonicus
  • chitosan
  • β-fructofuranosidase
  • Abstract The production of chitosan was conducted by a submerged culture of Asperjillus japonicus. During the fermentation using glucose as carbon source, adding 5 ppm of zinc ion resulted in cell growth up to 8.2 g/L, the chitosan with a molecular weight of 65 kDa and the degree of deacetylation of 96.79%. The maximum yield of chitosan at 43.6 mg/L and the chitosan content of 0.79% were achieved upon ferrous ion at 10 ppm. When sucrose was used as carbon source, the highest production of biomass at 6.4 g/L was obtained upon ferrous ion at 10 ppm. Adding 5 ppm of manganese ion resulted in chitosan up to 22.4 mg/L and a chitosan content of 0.46%. Adding 5 ppm of zinc ion or 10 ppm of ferrous ion gave the molecular weight of chitosan up to 49 kDa. The degree of deacetylation of chitosan was increased by adding ferrous ion. The activity of β-fructofuranosidase was enhanced by adding 5 ppm of zinc or manganese ion in the culture medium. The maximum activity was obtained upon manganese ion at 5 ppm.
    Advisor Committee
  • Dey-Chyi Sheu - advisor
  • none - co-chair
  • none - co-chair
  • Files indicate in-campus access at 5 years and off-campus access at 50 years
    Date of Defense 2007-07-20 Date of Submission 2007-08-14


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