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The defense date of the thesis is 2007-08-10
The current date is 2019-04-25
URN etd-0808107-161623 Statistics This thesis had been viewed 2318 times. Download 5 times. Author Tzu-Wei Chou Author's Email Address No Public. Department Bioengineering Year 2006 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 135 Title THE INVESTIGATION AND ANALYSIS OF ENVIRONMENTAL ADENOVIRUSES
IN COASTAL WATER
Keyword COASTAL WATER ADENOVIRUSES INVESTIGATION OF ENVIRONMENTAL INVESTIGATION OF ENVIRONMENTAL ADENOVIRUSES COASTAL WATER Abstract Enteric adenoviruses generally spread through faecal-oral route but the importance of viral transmission by water is probably underestimated. Thus, a reliable and sensitive method for detecting low concentrations of adenoviruses is needed. The objectives of this study was to: a)develop a rapid and efficiency virus concentration method； b)evaluate PCR and cell culture for detection of adenovirus from water samples; c)compare the cell culture method with the seminest-PCR method in coastal water samples. Three different charges of adsorbing membrane filters and two elution methods were used in this study. Nine concentration method combinations, adsorption on nitrocellulose membranes followed by an acid rinse elution consistently gave the highest recovery efficiencies. Total 158 coastal water samples total positive detection rate were increased to 19.0% (30/158) when we applied seminest-PCR. The concentration of E.coli and total coliforms showed significant correlation with the presence of adenoviruses in coastal waters. It suggested that current recreational water quality standards could reflect the virus contamination in coastal water.
Sequencing analysis of the coastal water samples revealed that presence of adenovirus group F (type 41), group B (type 3), and group D, and most isolates belonged to serotypes 3 (83.3%). As to clinical samples, it appeared that there were sporadic cases for group B (type 3), C (type 2) and F (type 41). We compare the nucleotide sequence of adenoviruses both from clinical and environmental isolates by phylogenetic analysis. The result showed that environmental isolates were clustered as clinical isolates. The present study proved that the nucleotide sequencing analysis for studying epidemiological relationships between strains involved in human infections and in coastal water.
We had established a sensitive and accurate method for screening of anti-adenovirus agents using the MTT method. A549 cell line were used for adenovirus type 41 and 3 infection and examined for the anti-ADV activities of several kinds of Allium vegetables. The antiviral concentration of 50% effectiveness(EC50) of shallot at adenovirus type 41 was 733.9mg/l and the EC50 of shallot at adenovirus type 3 was 1137.6mg/l. According to the results of this study,the mode of action of shallot is derived from inhibition at an early stage of viral replication after infection.
Advisor Committee Chien-Hsien Chen - advisor
Files Date of Defense 2007-07-02 Date of Submission 2007-08-10