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URN etd-0813104-150646 Statistics This thesis had been viewed 3403 times. Download 1251 times. Author Chin-Chu Ho Author's Email Address No Public. Department Bioengineering Year 2003 Semester 2 Degree Master Type of Document Master's Thesis Language English Page Count 106 Title Growth and Metabolism of Bifidobacteria in the Presence of High-Content Fructooligosaccharides Keyword β-fructofuranosidase oxidation-reduction potential (ORP) high-content fructooligosaccharides glucose oxidase bifidobacteria bifidobacteria glucose oxidase high-content fructooligosaccharides oxidation-reduction potential (ORP) β-fructofuranosidase Abstract Bifidobacteria can promote human health. Bifidobacteria are regularly found in human large intestine and colon, where digestible sugars such as glucose, sucrose and maltose, are not attainable. The investigation of enhancing the in vitro growth of bifidobacteria with such oligosaccharides, which is free of the metabolic interference of glucose and sucrose must be more significant than past research with adding commercial oligosaccharides, in that a large amount of digestible sugars are present.
High-content fructooligosaccharides are produced by a mixed-enzyme system with β-fructofuranosidase from Aspergillus japonicus and a commercial glucose oxidase as biocatalysts. Sucrose is catalyzed by β-fructofuranosidase, forming fructooligosaccharides and glucose, and this glucose is concurrently converted to gluconic acid which is then precipitated to calcium gluconate in solution through the catalysis of glucose oxidase. In such way and under an extreme aerobic condition made by aeration with 95 % (v/v) oxygen, up to 95 %, on a dry weight basis, of high-content fructooligosaccharides can be produced.
Five percent (w/v) of such high-content oligosaccharides are added to a 2-L bifidus culture in a 5-L jar fermenter. The fermentation is carried out under an anaerobic condition with gentle stirring. The optical density and the viable count of the fermentation medium are monitored. Furthermore, the utilization of each sugar component such as disaccharide, trisaccharide or tetrasaccharide is analyzed by HPLC throughout the fermentation process. Other parameters such as ORP, pH and the acetate to lactate ratio are also determined. During 72 h of fermentation, eight bifidobacteria including Bifidobacterium longum CCRC 14634, B. longum CCRC 14602, B. bifidum CCRC 14615, B. bifidum CCRC 11844, B. breve CCRC 11846, B. adolescentis CCRC 14607, B. adolescentis CCRC 14609 and B. pseudocatenulatum CCRC 15476 were investigated. B. adolescentis CCRC 14607 depleted 1-kestose completely at h 16 and B. longum CCRC 14602 consumed nystose completely at h 20, being the best strains among eight bifidobacteria. B. adolescentis CCRC 14609 produced the maximum lactic acid of 40.88 g/L and B. adolescentis CCRC 14607 produced the maximum acetic acid of 27.82 g/L. But B. bifidum CCRC 11844 and B. breve CCRC 11846 did not grow well and consumed fructooligosaccharides poorly. As FOS was being consumed, the ORP of the culture broth declined, until most of the FOS was depleted, and then it would increase very slowly. The decreasing of ORP reflected the consuming of FOS and the growth of bifidobacteria.
Advisor Committee Dey-chyi Sheu - advisor
Chi-tsai Lin - co-chair
Kow-jen Duan - co-chair
Files Date of Defense 2004-07-28 Date of Submission 2004-08-13