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The defense date of the thesis is 2009-08-17
The current date is 2019-05-23
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URN etd-0813109-212312 Statistics This thesis had been viewed 1614 times. Download 13 times. Author Shi-Rong Hong Author's Email Address No Public. Department Bioengineering Year 2008 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 83 Title Immobilization of DNA Probe on Carboxylated Carbon Nanotube for Detection of Vibrio parahaemolyticus Keyword interdigital electrode quartz crystal microbalance Vibrio parahaemolyticus Carbon nanotubes Carbon nanotubes Vibrio parahaemolyticus quartz crystal microbalance interdigital electrode Abstract Using the property of carbon nanotubes (CNTs) to design the biosensor that be more stable and sensitive. Surface treated CNTs by grafting on inorganic surface for single strand DNA immobilization.
In this study, we use two different systems to test. The ss-DNA can be convalently attached to CNTs by way of activated in acid oxidation conditions then PEI and GA chemistry immobilization. We can check the substance immobilization by the frequency shift using the QCM to detect.We can use the same grafting method to the interdigital electrode. When the DNA probe immobilization and hybridization, the resistance will be decrease. As a result, the concentrations of Vibrio parahaemolyticus change from 58.4 ng/ml to 1870 ng/ml, the resistance variate from 1.52 Ω to 3.04 Ω.
Advisor Committee Tsong-Rong Yan - advisor
none - co-chair
none - co-chair
Files Date of Defense 2009-07-29 Date of Submission 2009-08-17