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Title page for etd-0814106-151317


URN etd-0814106-151317 Statistics This thesis had been viewed 4988 times. Download 2356 times.
Author Jan-Yi Chang
Author's Email Address san_hou@ms96.url.com.tw
Department Bioengineering
Year 2005 Semester 2
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 67
Title THE EFFECT OF BANLANGEN (ISATIS INDIGOTICA FORT) ON THE EXPRESSION OF CYTOKINE-RELATED GENES
Keyword
  • Western blot.
  • Ban-Lan-Gen
  • real-time RT-PCR
  • array chip
  • cytokine-related genes
  • cytokine-related genes
  • array chip
  • real-time RT-PCR
  • Ban-Lan-Gen
  • Western blot.
  • Abstract Abstract
    The severe acute respiratory (SARS) was originated from China and became a pandemic disease in early 2003 and has claimed thousands of deaths worldwide.
    During the pandemic period, people in China believed that Banlangen (Isatis indigotica Fort) could cure or prevent the infection of SARS corona virus (SARS-CoV). However, the detail of the mechanism on how it functions on the treatment of SARS still remained unknown. Onepossibility is that Banlangen may influence cytokine signal pathway by regulating cytokine-related genes. In this study, we utilized RT-PCR to synthesize approximately 120-bp DNA fragment each for 248cytokine-related genes. The lung cancer cell line, A549, was treated with Banlangen, and the total mRNAs were then extracted. The expression difference of these gens in the presence and absence of Banlangen was determined by the array chip and was further by real-time RT-PCR. Our results revealed that Banlangen inhibited the expression of EphA1, EphA3, and DLK, whereas it enhanced the expression of Tyro3, IL30, and CXCL12 The expression of Tyro3 was also confirmed by Western bolt. These results will be further confirmed by animal study.
    Advisor Committee
  • Chien-Hsien Chen - advisor
  • Jan-Gowth Chang - co-chair
  • Lin, Ming-Tse - co-chair
  • Files indicate access worldwide
    Date of Defense 2006-06-22 Date of Submission 2006-08-14


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