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URN etd-0814106-151317 Statistics This thesis had been viewed 5048 times. Download 2356 times. Author Jan-Yi Chang Author's Email Address email@example.com Department Bioengineering Year 2005 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 67 Title THE EFFECT OF BANLANGEN (ISATIS INDIGOTICA FORT) ON THE EXPRESSION OF CYTOKINE-RELATED GENES Keyword Western blot. Ban-Lan-Gen real-time RT-PCR array chip cytokine-related genes cytokine-related genes array chip real-time RT-PCR Ban-Lan-Gen Western blot. Abstract Abstract
The severe acute respiratory (SARS) was originated from China and became a pandemic disease in early 2003 and has claimed thousands of deaths worldwide.
During the pandemic period, people in China believed that Banlangen (Isatis indigotica Fort) could cure or prevent the infection of SARS corona virus (SARS-CoV). However, the detail of the mechanism on how it functions on the treatment of SARS still remained unknown. Onepossibility is that Banlangen may influence cytokine signal pathway by regulating cytokine-related genes. In this study, we utilized RT-PCR to synthesize approximately 120-bp DNA fragment each for 248cytokine-related genes. The lung cancer cell line, A549, was treated with Banlangen, and the total mRNAs were then extracted. The expression difference of these gens in the presence and absence of Banlangen was determined by the array chip and was further by real-time RT-PCR. Our results revealed that Banlangen inhibited the expression of EphA1, EphA3, and DLK, whereas it enhanced the expression of Tyro3, IL30, and CXCL12 The expression of Tyro3 was also confirmed by Western bolt. These results will be further confirmed by animal study.
Advisor Committee Chien-Hsien Chen - advisor
Jan-Gowth Chang - co-chair
Lin, Ming-Tse - co-chair
Files Date of Defense 2006-06-22 Date of Submission 2006-08-14