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Title page for etd-0815106-130111


URN etd-0815106-130111 Statistics This thesis had been viewed 1821 times. Download 6 times.
Author Chih-Wei Chi
Author's Email Address No Public.
Department Bioengineering
Year 2005 Semester 2
Degree Master Type of Document Master's Thesis
Language English Page Count 79
Title Effect of Urea Concentration on Production of L-Lactic Acid and Chitosan by Culture of Rhizopus oryzae
Keyword
  • urea
  • Rhizopus oryzae
  • L-lactic acid
  • chitosan
  • chitosan
  • L-lactic acid
  • Rhizopus oryzae
  • urea
  • Abstract During L-lactic acid fermentation by culture of Rhizopus oryzae ATCC 9363, the production of biomass and chitosan was affected by the urea concentration. When fermentation was carried out in a 5-L stirred fermenter by adding calcium hydroxide to control the pH at 6.0, biomass increased from 4.35 g/L to 7.25 g/L as urea from 2.5 to 10.0 g/L was used as N-source; meanwhile, the produced chitosan increased from 0.290 g to 0.841 g. The highest yield of L-lactic acid was 101.3 g/L as 5.0 g/L of urea was used. Upon 5.0 g/L of urea as N-source and 120 g/L of glucose as substrate, 8.15 g of biomass and the highest yield of chitosan at 1.362 g were obtained when pH was controlled at 6.0 by adding a mixture of sodium hydroxide and sodium bicarbonate.
    Advisor Committee
  • Dey-Chyi Sheu - advisor
  • Files indicate in-campus access at 2 years and off-campus not accessible
    Date of Defense 2006-07-20 Date of Submission 2006-08-15


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