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The defense date of the thesis is 2006-08-15
The current date is 2019-02-18
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URN etd-0815106-130111 Statistics This thesis had been viewed 1821 times. Download 6 times. Author Chih-Wei Chi Author's Email Address No Public. Department Bioengineering Year 2005 Semester 2 Degree Master Type of Document Master's Thesis Language English Page Count 79 Title Effect of Urea Concentration on Production of L-Lactic Acid and Chitosan by Culture of Rhizopus oryzae Keyword urea Rhizopus oryzae L-lactic acid chitosan chitosan L-lactic acid Rhizopus oryzae urea Abstract During L-lactic acid fermentation by culture of Rhizopus oryzae ATCC 9363, the production of biomass and chitosan was affected by the urea concentration. When fermentation was carried out in a 5-L stirred fermenter by adding calcium hydroxide to control the pH at 6.0, biomass increased from 4.35 g/L to 7.25 g/L as urea from 2.5 to 10.0 g/L was used as N-source; meanwhile, the produced chitosan increased from 0.290 g to 0.841 g. The highest yield of L-lactic acid was 101.3 g/L as 5.0 g/L of urea was used. Upon 5.0 g/L of urea as N-source and 120 g/L of glucose as substrate, 8.15 g of biomass and the highest yield of chitosan at 1.362 g were obtained when pH was controlled at 6.0 by adding a mixture of sodium hydroxide and sodium bicarbonate. Advisor Committee Dey-Chyi Sheu - advisor
Files Date of Defense 2006-07-20 Date of Submission 2006-08-15