首頁 > 網路資源 > 大同大學數位論文系統

Title page for etd-0817107-144652


URN etd-0817107-144652 Statistics This thesis had been viewed 2253 times. Download 19 times.
Author Wei-guo Gu
Author's Email Address No Public.
Department Bioengineering
Year 2006 Semester 2
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 94
Title Fabrication and properties of PDMS-based flow-through PCR device
Keyword
  • poly(dimethylsiloxane)
  • Polymerase chain reaction
  • Pluronic
  • neat silane
  • neat silane
  • Pluronic
  • Polymerase chain reaction
  • poly(dimethylsiloxane)
  • Abstract Polymerase chain reaction (PCR) utilizes DNA polymerase to amplify specific DNA fragment. The reaction is carried out under three different reaction temperatures, and the amplified DNA product can be used in subsequent analysis and research. In this thesis, a PDMS-based flow-though PCR device was fabricated using micro-fabrication techniques. The flow channel (50 μm deep) plate and a 1 mm-thick flat back plate were fabricated using PDMS, then the two plates were bonded after oxygen plasma treatment. A thin-film chromium resistive heater was manufactured on Corning 1737 glass substrate. The PCR device was assembled simply by placing the PDMS flow channel on the top of the glass heating chip. The cDNA of D-amino acid oxidase (from Trigonopsis variabilis) was used as the DNA template. The effects of flow rate and cross contamination on the efficiency of the PCR device were studied. In addition, the effects of surface modification by BSA, Pluronic® F-68, Pluronic® F-127, and neat silane were discussed.
    Advisor Committee
  • Chi-yang Yu - advisor
  • Files indicate in-campus access at one year and off-campus not accessible
    Date of Defense 2007-07-24 Date of Submission 2007-08-17


    Browse | Search All Available ETDs