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The defense date of the thesis is 2007-08-17
The current date is 2019-07-19
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URN etd-0817107-144652 Statistics This thesis had been viewed 2304 times. Download 19 times. Author Wei-guo Gu Author's Email Address No Public. Department Bioengineering Year 2006 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 94 Title Fabrication and properties of PDMS-based flow-through PCR device Keyword poly(dimethylsiloxane) Polymerase chain reaction Pluronic neat silane neat silane Pluronic Polymerase chain reaction poly(dimethylsiloxane) Abstract Polymerase chain reaction (PCR) utilizes DNA polymerase to amplify specific DNA fragment. The reaction is carried out under three different reaction temperatures, and the amplified DNA product can be used in subsequent analysis and research. In this thesis, a PDMS-based flow-though PCR device was fabricated using micro-fabrication techniques. The flow channel (50 μm deep) plate and a 1 mm-thick flat back plate were fabricated using PDMS, then the two plates were bonded after oxygen plasma treatment. A thin-film chromium resistive heater was manufactured on Corning 1737 glass substrate. The PCR device was assembled simply by placing the PDMS flow channel on the top of the glass heating chip. The cDNA of D-amino acid oxidase (from Trigonopsis variabilis) was used as the DNA template. The effects of flow rate and cross contamination on the efficiency of the PCR device were studied. In addition, the effects of surface modification by BSA, Pluronic® F-68, Pluronic® F-127, and neat silane were discussed. Advisor Committee Chi-yang Yu - advisor
Files Date of Defense 2007-07-24 Date of Submission 2007-08-17