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URN etd-0817112-120945 Statistics This thesis had been viewed 1367 times. Download 9 times. Author Ruo-Yu Chen Author's Email Address No Public. Department Bioengineering Year 2011 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 114 Title THE STUDY OF DOUBLE-LAYER SURFACE MODIFICATION OF POROUS EPTFE FILMS BY PLASMA TREATED AND THE EFFECTS ON THE GROWTH OF CELLS Keyword Human Umbilical Vein Endothelial Cells CCD966SK fibroblasts ePTFE ePTFE CCD966SK fibroblasts Human Umbilical Vein Endothelial Cells Abstract Expanded polytetrafluoroethylene (ePTFE) films, was treated with argon plasma to improre the hydrophilicity.
To create active site on the surface of ePTFE films, the coupling reaction with poly(ethylene glycol) methacrylate (PEGMA) or acrylamid (AAm) was initiated with UV. The contact angle was improved from 135°to 0°for modified ePTFE film with grafting poly(ethylene glycol) methacrylate (PEGMA；10wt%, and 30 minutes). The surface properties of ePTFE-g-PEGMA films and ePTFE-g-AAM films were also characterized by electron spectroscopy for chemical analysis, fourier transform infrared spectroscopy and scanning electron microscope. Peptide cRRE and collagen was crosslinked with (N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide)(EDC 5w%) on ePTFE-g-PEGMA films and ePTFE-g-AAM films. Human umbilical vein endothelial cells on one side of the ePTFE-g-AAm fixed polypeptide chain cRRE and ePTFE-g-AAm fixed collagen of the surface of HUVECs attached to the better,on the other hand the surface of human fibroblasts there is no better attachment rate of change menbrane, increasing the culture days to one weeks, better attached to the ePTFE-g-AAm and ePTFE-g-PEGMA fixed collagen rate in order to establish this modification in the field of biomedical applications. Different cells have different growth cycles, some the cell growth pay attention to develop the number of days in the double-sided .That can be enhance the biocompatibility of the film.
Advisor Committee Chung-Yih Wang - advisor
Files Date of Defense 2012-07-13 Date of Submission 2012-08-17