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URN etd-0821108-134749 Statistics This thesis had been viewed 4188 times. Download 1460 times. Author Yi-Chien Ho Author's Email Address No Public. Department Bioengineering Year 2007 Semester 2 Degree Ph.D. Type of Document Doctoral Dissertation Language English Page Count 179 Title TUDY ON THE HEPATOPROTECTION OF ANTRODIA CINNAMOMEA AND ANTI-HEPATOMA ACTIVITIES OF ETHANOLIC EXTRACTS FROM HOPS AND HALOFERAX MEDITERRANEI Keyword hepatocellular carcinoma apoptosis xanthohumol Hepatoprotection Antrodia cinnamomea triterpenoids polysaccharide anti-hepatoma activity Haloferax mediterranei Haloferax mediterranei anti-hepatoma activity polysaccharide triterpenoids Antrodia cinnamomea Hepatoprotection xanthohumol apoptosis hepatocellular carcinoma Abstract The hepatocellular carcinoma (HCC) is one of the most prevalent cancers worldwide, with more than 1 million new cases estimated annually. Therefore, preventing HCC and HCC therapy is a major research around the world. In the past researches, some herbal extracts used traditionally in hepatic diseases and some bioactive compounds extracted from the secondary metabolites of plant and microorganism have extensive biological activities, such as anti-hepatitis B virus, antioxidation, immunomodulating and anticancer effects.
In the first instance, the objective of this study was to produce Antrodia cinnamomea mycelia and its metabolites on a large scale, such as extracellular polysaccharides (EPS), intracellular polysaccharides (IPS), and triterpenoids. The hepatoprotective effects of the water extract from the mycelia of A. cinnamomea (WAC) were also evaluated in vitro using ethanol-induced cytotoxicity on AML12 hepatocytes. The cytotoxicity to AML12 cells induced by 300 mM ethanol was reduced effectively by adding 500 mg/L of WAC.
Secondly, we also investigated the inhibitory effects of xanthohumol on the human HCC cell lines. This result indicates that normal mouse hepatocyte cell line had more resistance to xanthohumol than HCC cell lines. Besides, the inhibitory effects of xanthohumol on human HCC cell lines were attributed to apoptosis as indicated in the results of flow cytometry, fluorescent nuclear staining and electrophoresis of oligonucleosomal DNA fragments.
Finally, the anti-hepatoma effects of the ethanol-extracted red pigments from Haloferax mediterranei (hmERP) were investigated. The hmERP was composed mainly of bacterioruberin, a carotenoid derived from lycopene, and showed a more powerful anti-hepatoma activity on HepG2 cells than lycopene. When HepG2 was exposed to 0.5 mg/L hmERP, the apoptosis-associated phosphatidyl serine redistribution, chromatin condensation, DNA fragmentation, and apoptotic body formation were observed. By flow cytometry analysis, hmERP induced the apoptotic cell fraction of HepG2 without increasing the necrotic cell fraction.
Advisor Committee Ming-Tse Lin - advisor
C-Will Chen - co-chair
Chi-Hsien Liu - co-chair
Kow-Jen Duan - co-chair
Lon-Sing Kan - co-chair
Tsong-Rong Yan - co-chair
Files Date of Defense 2008-06-17 Date of Submission 2008-08-21