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Title page for etd-0822108-115511


URN etd-0822108-115511 Statistics This thesis had been viewed 2572 times. Download 38 times.
Author Tai-Chih Su
Author's Email Address No Public.
Department Bioengineering
Year 2007 Semester 2
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 94
Title EVALUATION OF THE PERMEABILITY OF DIFFERENT LIPOSOME-ENCAPSULATED LOVASTATIN BY APPLICATION OF THE CACO-2 MODEL
Keyword
  • CACO-2
  • Liposome
  • Permeability of different
  • Permeability of different
  • Liposome
  • CACO-2
  • Abstract Caco-2 cells cultured on polycarbonate membrane can be spontaneously differentiated to close cell monolayer which is the same as gastrointestinal epithelial cells. Caco-2 cells as a model system can conquer the defects of using a lot of drugs for absorption and metabolism of animals. Hence, they can be used for sifting application of medicine which is absorbed in gastrointestinal tracts. Liposomes have some properties, such as good biocompatibility biodegradation, anti-immunization, and can be used for encapsulation of fat-soluble and water-soluble drug. Hence, they can be good carriers for drug delivery. In this study, liposomes for lavastatin was made by DMPC (Dimyristoyl-sn-Glycero-3-Phosphocholine) or EYPC (Egg-yolk L-α-Phosphatidylcholine) and permeation test of lovastatin by Caco-2 as a model system was studied. The vesicle volume distribution, entrapment efficiency, and cytotoxicity were analyzed and drug permeation studies through the Caco-2 cell were studied. Lipsomes were co-cultured with Caco-2 cells and significant cytotoxicity was not found. It is found that the entrapment efficiency of DMPC liposomes is 95.2 ± 0.54 (%), and EYPC liposomes is 86.76 ± 4.71 (%) when the lipids and the drugs got a ratio of 1:0.25 (mol) via HPLC Analyzer. The drug permeation of DMPC and EYPC liposomes through a monolayer made by Caco-2 cells are 5.62 and 5.9 times better than the permeation of pure drug . The result was tested using the lipsomes made by lovastatin and pure drug of lovastatin going through a monolayer made by Caco-2 cells.
    Advisor Committee
  • Trong-Rong Yan - advisor
  • Chung-Yih Wang - co-chair
  • yuan- syun syu - co-chair
  • Files indicate in-campus access only
    Date of Defense 2008-07-24 Date of Submission 2008-08-23


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