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The defense date of the thesis is 2006-08-23
The current date is 2019-03-23
URN etd-0823106-151917 Statistics This thesis had been viewed 2421 times. Download 5 times. Author Wei-tsung Hsu Author's Email Address No Public. Department Bioengineering Year 2005 Semester 1 Degree Master Type of Document Master's Thesis Language English Page Count 98 Title THE DISTINCTION AND ANALYSIS OF CHITINASES FROM HUMAN PLASMA Keyword chitinases human plasma human plasma chitinases Abstract Chitinolytic enzymes exist in various species in nature. As a glycosyl hydrolases, they can catalyze the hydrolysis of chitin, the second most abundant polysaccharide on earth. Chitinolytic enzymes are necessary in the development of metabolism for arthropoda and fish. In human, there are many kinds of chitinolytic enzymes and chitinase-like proteins. Their physiological functions are yet appreciated.
In this study, chitinase and related proteins from human plasma were identified using liquid chromatography. In one method, Blue Sepharose 6 affinity liquid chromatography and DEAE Sepharose ion exchange liquid chromatography were used to separate proteins from human plasma. A protein with molecular weight of 150 kDa, could be identified by western blot using ChBD antiserum. In other way, chitin affinity liquid chromatography and DEAE Sepharose ion exchange liquid chromatography were used to separate proteins from human plasma. A protein with molecular weight of 30 kDa was identified with ability to catalyze the hydrolysis of glycol chitin by in-situ activity staining on SDS-PAGE.
Advisor Committee Chun Yi Liau - advisor
Ming-tse Lin - advisor
Chao-hsiung Lin - co-chair
I-ching Kuan - co-chair
Files Date of Defense 2006-06-19 Date of Submission 2006-08-23