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Title page for etd-0823106-151917


URN etd-0823106-151917 Statistics This thesis had been viewed 2421 times. Download 5 times.
Author Wei-tsung Hsu
Author's Email Address No Public.
Department Bioengineering
Year 2005 Semester 1
Degree Master Type of Document Master's Thesis
Language English Page Count 98
Title THE DISTINCTION AND ANALYSIS OF CHITINASES FROM HUMAN PLASMA
Keyword
  • chitinases
  • human plasma
  • human plasma
  • chitinases
  • Abstract Chitinolytic enzymes exist in various species in nature. As a glycosyl hydrolases, they can catalyze the hydrolysis of chitin, the second most abundant polysaccharide on earth. Chitinolytic enzymes are necessary in the development of metabolism for arthropoda and fish. In human, there are many kinds of chitinolytic enzymes and chitinase-like proteins. Their physiological functions are yet appreciated.
    In this study, chitinase and related proteins from human plasma were identified using liquid chromatography. In one method, Blue Sepharose 6 affinity liquid chromatography and DEAE Sepharose ion exchange liquid chromatography were used to separate proteins from human plasma. A protein with molecular weight of 150 kDa, could be identified by western blot using ChBD antiserum. In other way, chitin affinity liquid chromatography and DEAE Sepharose ion exchange liquid chromatography were used to separate proteins from human plasma. A protein with molecular weight of 30 kDa was identified with ability to catalyze the hydrolysis of glycol chitin by in-situ activity staining on SDS-PAGE.
    Advisor Committee
  • Chun Yi Liau - advisor
  • Ming-tse Lin - advisor
  • Chao-hsiung Lin - co-chair
  • I-ching Kuan - co-chair
  • Files indicate not accessible
    Date of Defense 2006-06-19 Date of Submission 2006-08-23


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