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URN etd-0824104-182901 Statistics This thesis had been viewed 3534 times. Download 1761 times. Author Ya-ting Shu Author's Email Address No Public. Department Bioengineering Year 2004 Semester 2 Degree Master Type of Document Master's Thesis Language English Page Count 118 Title EFFECT OF HIGH-CONTENT ISOMALTOOLIGOSACCHARIDES ON THE GROWTH OF BIFIDOBACTERIA Keyword probiotics high-content isomaltooligosaccharides bifidobacteria bifidobacteria high-content isomaltooligosaccharides probiotics Abstract Bifidobacteria can promote human health. Bifidobacteria are regularly found in human large intestine and colon, where digestible sugars such as glucose, sucrose and maltose, are not attainable. Isomaltooligosaccharides (IMO), a function sugar, was made from maltose via a reaction catalyzed by glucosyltransferase. IMO is preprobiotic, being able to enhance the growth of bifidobacteria. High-content IMO can be produced, by treating commercial IMO syrup with yeast, thereby digestible sugar including maltose and glucose are depleted. In this way, the content of IMO increases from 58% to 99% on a dry weight basis. In a 2-L jar fermenter, bifidobacteria were cultured in 1.3 L broth in the presence of 5% (w/v) high-content IMO. The fermentation conditions were as following: anaerobic culture, overlaid with a layer of liquid paraffin; stir rate, 100rpm; temperature, 37°C. During fermentation, turbidity, viable count, ORP, pH, acetate, lactate and sugar components were determined periodically. During 72 h of fermentation, most of the bifidobacteria used in this study metabolize primarily panose, tertesaccharides and isomaltose and thus produced G and IG2. B. adolescentis CCRC 14609 consumed IMO much faster than other strains did and produced the maximum lactic acid of 37.41 g/L. But B. bifidum CCRC 11844 and B. breve CCRC 11846 did not grow well and consumed isomaltooligosaccharides poorly.
The present investigation using such oligosaccharides which was free of digestible sugars was much more significant than past research, in which oligosaccharides accompanied with large amounts of digestible sugars were used. The utilization of various IMO components by eight bifidobacteria was clearly understood.
Advisor Committee Dey-Chyi Sheu - advisor
Files Date of Defense 2004-07-28 Date of Submission 2004-08-24