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URN etd-0824106-080651 Statistics This thesis had been viewed 3798 times. Download 1184 times. Author Shih-Feng Hsueh Author's Email Address No Public. Department Materials Engineering Year 2005 Semester 2 Degree Master Type of Document Master's Thesis Language English Page Count 119 Title The Study of Immobilization of Glucose Oxidase on Inorganic Surface for Biosensor Keyword glucose oxidase interdigital electrode biosensor biosensor interdigital electrode glucose oxidase Abstract Many research workers attempt to fabricate sensitive, selective, reliable and low cost glucose sensors. Among these sensors, amperometric electrodes based on the immobilized glucose oxidase (GOD) have attracted considerable interest due to their simple alternative analytical systems. Because sensitive layer always is coating in conductive electrode. In this study, we used the hexamethyldisilazane (HMDSZ) to plasma deposit organic silicon-containing thin films on the surface of interdigital electrode substrates in order to make an interface layer between the inorganic materials and organic molecules. The UV-light induced surface grafting polymerization of acrylamide (AAm) can provide useful functional NH2-groups sites for binding glucose oxidase (GOD). Hence glutaraldehyde (GA) as cross–linking agent can connect the grafting polymer and the GOD enzyme. In the FTIR spectra, Si-O-C, Si-O-Si Si-CH2-Si, Si-N-Si, and Si-CH3 groups were found after plasma deposition. XPS analysis shows PEI treatment could raise amount of GOD immobilization with GA. Experiment results indicated that GOD can be immobilization effectively on interdigital electrode substrate. Advisor Committee Ko-Shao Chen - advisor
Mu-Rong Yang - co-chair
Shun-Fu, Lin - co-chair
Files Date of Defense 2006-07-01 Date of Submission 2006-08-24