||There are many report have exhibit about Lactic acid bacteria, which can improve intestine discomfortable and Infectious diarrhea, and also improving immunization system. In cancer prevent, it’s already prove that can decrease some types of cancer. In this study, the five lactobacillus strains isolated from local Taiwanese fermented vegetables were used to fermented with different concentrations of sake lees extract (use different solvent extract, 10, 1, 0.1 mg/ml), and the supernatant filtrates were used to keep skin whitening and inhibition cancer cell growth. According to the results, to inhibit Hepatocellular Carcinoma and colon cells growth, cell survivability well decrease when enhancing concentrations of sake lees extract, and the supernatant filtrates from Lb. plantanum E38 could obviously inhibit the mushroom tyrosinase activity of 54 % , and decrease melanin contents of B16-F0 cell line of 54 % , equal to 3.4 μg/ml melanin. DPPH radical scavenging effect well decrease to reduce concentrations of sake lees extract for the most part. The tyrosinase inhibitory compounds contained in supernatant filtrates identify as linoleic acid using HPLC. The cancer inhibitor maybe inoleic acid isomers, presently have many report about inoleic acid isomers can anti-inflammatory and anticarcinogenic properties. Therefore, the fermentation products from sake lees by the lactobacilli will be very useful for application in the cosmetic and anticarcinogenic medicine.