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URN etd-0826109-133811 Statistics This thesis had been viewed 2158 times. Download 1598 times. Author Chih-Yung Chiang Author's Email Address No Public. Department Bioengineering Year 2008 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 132 Title Different chemical structure of polyhydroxyalkaoates produced from Haloferax mediterranei by using different hydrocarbon precursors Keyword polyhydroxyalkaoates Haloferax mediterranei Haloferax mediterranei polyhydroxyalkaoates Abstract In order to decrease the demand of petroleum-based plastic,
therefore developing the substitive materials that could decompose
naturally and are harmless to environments is becoming one of the most
important green technologies. Extreme halophiles, Haloferax
mediterranei, could live in the environment of high concentrations of
NaCl, and not only could be cultured for long period of time without the
risk of microbial contamination, but also produce biodegraded
polyhydroxyalkanoates (PHA). The structures of most of PHA are hard
and brittle materials composed mostly by 3-hydroxybutyrate (3HB)
monomer. The increasing of 3-hydroxyvalerate (3HV) monmer within
PHA structure could improve its elongation and toughness. In this
research, we find when we use the glucose as the carbon source for
cultivating H. mediterranei, we can only get PHA of 4.8 % 3HV. If using
the agricultural wastes like ground bagasses as the carbon source to
culture H. mediterranei , we still get PHA of low percentage (5.3 %) 3HV.
By using glucose or soybean oil hydrolyte as the carbon sources and
adding precursors of valerate (0.5 g/L) at cucultivation time of 34 hours
can promote producing PHA of 26.0 % 3HV (in case of glucose) and 26.7
% 3HV (in case of soybean oil hydrolyte). By adding 1 g/L sodium
valerate at cucultivation time of 34 hours can get PHA of 35.2 % 3HV (in
case of glucose) and 36.5 % 3HV (in case of soybean oil hydrolyte).
When adding mixture of valerate (0.5 g/L) and sodium valerate (1 g/L) as
the precursors at cucultivation time of 34 hours, we can get 3HV of 37.4±
7.2 % (in case of glucose) ; we can get 3HV of 48.2±29.6 % (in case of
soybean oil hydrolyte). In the study, we also find that sodium valerate can
promote H. mediterranei growing. When increasing sodium valerate form
0.5 g/L to 8 g/L in the culture medium, we can get the maximum cell
concentration of OD520nm form 11.3 to 33.1
Advisor Committee Chen, C. Will - advisor
Files Date of Defense 2009-07-31 Date of Submission 2009-08-26