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URN etd-0827108-111155 Statistics This thesis had been viewed 2723 times. Download 2540 times. Author Yi-Hui Hsu Author's Email Address No Public. Department Bioengineering Year 2007 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 77 Title The separation and analysis of Red Pigments Extracted from Haloferax mediterranei by Liquid Chromatography and Mass spectrum Keyword Mass spectrum Haloferax mediterranei Haloferax mediterranei Mass spectrum Abstract Haloferax mediterranei is used in this study as an extremely
halophilic archaebacterium for red pigment (hmERP) production. In
this study, we investigate the composition, characteristic structure and
molecular weight of components in hmERP. The optical properties of
hmERP were analyzed by UV/VIS Spectrophotometer, and shown that
the absorption wavelength of hmERP are 527, 494, 468, 388 and 371
nm with maximum wavelength at 494 nm. We also found that the
absorption wavelength of hmERP decayed regularly under light
irradiation. From the infrared absorption spectrum, the molecular
structure of hmERP contain hydroxy (-OH) and C=C double bond.
After separating by reversed-phase High Performance Liquid
Chromatography (HPLC) at C18 column with 70% acetone and 30%
water as the mobile phase, the hmERP contains at least six
compounds. The molecular weight 741.6(M+H)+ of peak A (major
peak with shortest retention time) in hmERP was obtained by
LC-APCI-MS. Based on the IR spectrum, molecular structure and
molecular weigh analysis, we conclude that component in peak A of
hmERP is same as Bacterioruberin (a C50 carotenoid derivative).
Advisor Committee (Chen, C. Will) - advisor
(Lin, Ming-Tse) - co-chair
Files Date of Defense 2008-07-31 Date of Submission 2008-08-27