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Title page for etd-0827108-111155


URN etd-0827108-111155 Statistics This thesis had been viewed 2772 times. Download 2540 times.
Author Yi-Hui Hsu
Author's Email Address No Public.
Department Bioengineering
Year 2007 Semester 2
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 77
Title The separation and analysis of Red Pigments Extracted from Haloferax mediterranei by Liquid Chromatography and Mass spectrum
Keyword
  • Mass spectrum
  • Haloferax mediterranei
  • Haloferax mediterranei
  • Mass spectrum
  • Abstract Haloferax mediterranei is used in this study as an extremely
    halophilic archaebacterium for red pigment (hmERP) production. In
    this study, we investigate the composition, characteristic structure and
    molecular weight of components in hmERP. The optical properties of
    hmERP were analyzed by UV/VIS Spectrophotometer, and shown that
    the absorption wavelength of hmERP are 527, 494, 468, 388 and 371
    nm with maximum wavelength at 494 nm. We also found that the
    absorption wavelength of hmERP decayed regularly under light
    irradiation. From the infrared absorption spectrum, the molecular
    structure of hmERP contain hydroxy (-OH) and C=C double bond.
    After separating by reversed-phase High Performance Liquid
    Chromatography (HPLC) at C18 column with 70% acetone and 30%
    water as the mobile phase, the hmERP contains at least six
    compounds. The molecular weight 741.6(M+H)+ of peak A (major
    peak with shortest retention time) in hmERP was obtained by
    LC-APCI-MS. Based on the IR spectrum, molecular structure and
    molecular weigh analysis, we conclude that component in peak A of
    hmERP is same as Bacterioruberin (a C50 carotenoid derivative).
    Advisor Committee
  • (Chen, C. Will) - advisor
  • (Lin, Ming-Tse) - co-chair
  • Files indicate in-campus access immediately and off-campus access at one year
    Date of Defense 2008-07-31 Date of Submission 2008-08-27


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