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URN etd-0829108-144845 Statistics This thesis had been viewed 2859 times. Download 554 times. Author Kai-Ho Mak Author's Email Address No Public. Department Bioengineering Year 2007 Semester 2 Degree Master Type of Document Master's Thesis Language English Page Count 111 Title Immobilization of Lipase from Pseudomonas cepacia onto Magnetic Nanoparticles Keyword Biodiesel Magnetic nanoparticles Lipase Immobilization Immobilization Lipase Magnetic nanoparticles Biodiesel Abstract Biodiesel, synthesized from oils/fats and alcohols with catalyst, recently has become an alternative source of renewable fuels. In addition to acidic and alkaline catalysts, biodiesel production by immobilized lipase possessed advantage such as mild reaction conditions ease product separation and using waste oil as raw materials. Magnetic nanoparticles high specific surface area was easily separated from reaction mixture when applying a magnetic field. In the present study, optimum condition of immobilized Pseudomonas cepacia lipase (PcL) onto magnetic nanoparticles and characteristic of immobilized lipase were examined. Production of biodiesel by this immobilized PcL was also evaluated.
The results showed that the Fe3O4 magnetic nanoparticles were obtained by coprecipitating FeCl3 and FeCl2 ions in an ammonia solution. The analyses of transmission electron microscopy (TEM) and X-ray diffraction (XRD) showed that the resultant magnetic nanoparticles were pure Fe3O4 with a mean diameter of 16 nm compared of Fe3O4. PcL magnetic nanoparticles were via carbodiimide (CDI) activation and Fourier transform infrared (FTIR) spectroscopy confirmed the binding of PcL onto Fe3O4 magnetic nanoparticles. When PcL was immobilized on the Fe3O4 at a weight ratio of 0.1, cross-linked with 0.03g/ml CDI in phosphate buffer (pH 8.0) containing 0.1M NaCl at 4℃ for 30 min, the higher amount the bound lipase reached ca. 90% and the corresponding activity recovery was 63%. The optimum temperature and pH for immobilized lipase was similar to those of free one. Thermal stability and storage stability were significantly improved after immobilization and reusability was also found. Moreover, molar yield of 93 mol% for biodiesel production after 72 hr was obtained by using immobilized PcL onto Fe3O4 magnetic nanoparticles.
Advisor Committee Shiow-Ling Lee - advisor
Cheng-chun Chou - co-chair
Chi-yang Yu - co-chair
Files Date of Defense 2008-07-30 Date of Submission 2008-08-29