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The defense date of the thesis is 2016-08-31
The current date is 2019-05-19
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URN etd-0829116-164105 Statistics This thesis had been viewed 504 times. Download 0 times. Author Hao-Chan Hsu Author's Email Address No Public. Department Materials Engineering Year 2015 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 72 Title The Study of Catechol Conjugated Chitosan Coated Multi-Shell Calcium Phosphate Nanocarrier for Gene Delivery Keyword calcium phosphate chitosan catechol gene delivery carrier gene delivery carrier catechol chitosan calcium phosphate Abstract Calcium phosphate (CAP) based gene carrier preserve an attractive option for gene transfection due to their high biocompatibility and biodegradability property. Although there is great potential for the use of CAP in the development of gene delivery systems, the uncontrollable growth of CAP crystal and low protection of pDNA ability make it difficult to deliver genetic materials into cells.
In this study, the transfection efficiency is optimized by varying the stoichiometry (Ca/P molar ratio) of the double concentric layers of calcium phosphate (DCP) and catechol-conjugated DCP with surface modification by chitosan (Chi). Our results indicate that the increase of DCP (Core Ca/P ratio was 700, shell Ca/P ratio was 130) will restrict the size (108 nm) of CAP crystal growth.
Moreover, A bioadhesive molecule from marine mussels, 3,4-dihydroxy-L-phenylalanine (Dopa), is successfully used to bridge inorganic Ca2+ of DCP and organic Chi-functionalized DCP (Chi-D-DCP). The positive surface charge(+36.2mV) of Chi-D-DCP not only improves the binding affinity with plasmid DNA (pDNA) but also obtains better condensation efficacy when compared to Chi-DCP. The efficiency of pDNA protection could be considerably increased by adding another layer of Chi-D on the DCP surface, resulting in successfully incorporating pDNA into the nanocarrier and preventing its degradation within the cell by lysosomes. It is suggested that Chi-D-DCP nanocarrier has good DNase resistant 95% effect, better biocompatibility, and in the Hela cell 32.4% transfection efficary for In vitro. This stabilized CAP-based gene delivery platform could be considered a promising candidate as gene delivery carrier.
Advisor Committee Hsi-Chin Wu - advisor
Jui-Sheug Sun - co-chair
Tzu-Wei Wang - co-chair
Files Date of Defense 2016-07-27 Date of Submission 2016-08-31