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URN etd-0831110-171337 Statistics This thesis had been viewed 2477 times. Download 1761 times. Author Shou-Yu Hsu Author's Email Address No Public. Department Bioengineering Year 2009 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 81 Title Ethanol production from cellulose and orange peel using thermotolerant yeasts Keyword orange peel thermotolerant yeasts fermentation cellulose cellulose fermentation thermotolerant yeasts orange peel Abstract In this study, we selected thermotolerant yeasts to produce ethanol from cellulose in the presence of cellulase. This study falled into two sections. The first section was try to find the thermotolerant yeast, which can grow at 40 – 45 ℃. The other section was using the thermotolerant yeast to produce ethanol from cellulose and orange peel waste by simultaneous saccharification and fermention.
Among the fifty strains of thermotolerant yeast that we screened, the most thermotolerant were the Kluyveromyces marxianus genus yeasts. From those Kluyveromyces marxianus genus yeasts, we selected a yeast strain and called it “K21”. Ethanol production was carried out using 7.5 % (w/v) alpha-cellulose as substrate in the percent of AccelleraseTM 1000 and K21 yeast cells by simultaneous saccharification and fermentation (SSF) at 42, and 45 ℃, respectively.
The results indicate that the viability at 42 ℃ was higher than at 45 ℃, and the maximum ethanol concentration were 30 g/L and 45g/L, at 42 ℃ and 45 ℃, respectively.
The orange peel was dried in the 70 ℃ oven and then was ground into powders. Ethanol production was carried out in jar fermenter using orange peel as raw material in the presence of AccelleraseTM 1000, pectinase and K21 yeast cells by SSF. The results indicate that 10 % (w/v) orange peel resulted an ethanol concentration of 17 g/L, and the 20 % (w/v) orange peel led to an ethanol concentration of 33 g/L, respectively.
Advisor Committee Kow-Jen Duan - advisor
Dey-Chyi Sheu - co-chair
Wen-chien Lee - co-chair
Files Date of Defense 2010-07-16 Date of Submission 2010-09-01