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URN etd-0831110-214618 Statistics This thesis had been viewed 3135 times. Download 2326 times. Author Shiau-Chi Chen Author's Email Address No Public. Department Bioengineering Year 2009 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 90 Title Production of high-content isomaltooligosaccharides by coimmobilized transglucosidase and yeast cell Keyword Calcium alginate Immobilized cell and enzyme Isomaltooligosaccharides Transglucosidase Transglucosidase Isomaltooligosaccharides Immobilized cell and enzyme Calcium alginate Abstract Isomaltooligosaccharides（IMO）produced through the catalytic reaction of transglucosidase on maltose contain glucose and several oligosaccharides which have at least one α-D-(1,6) glucosidic linkage. IMO is a functional food ingredient which has great potential to improve the quality of many foods. IMO is a prebiotic, because it is non-digestible and can stimulate the proliferation of bifidobacteria in human intestine. High content IMO produced by removing byproduct glucose from IMO is receiving increasing interest due to its low calories, no cariogenicity and safety for diabetics.
This work fell into two parts. In part I the optimal concentration of crosslinker EDC for the immobilization of transglucosidase on alginate was investigated. In part II the temperature tolerant yeast was immobilized using the enzyme-immobilized alginate, forming beads of calcium alginate. In this dual-biocatalyst system, high content IMO was obtained from maltose due to the byproduct glucose being removed.
The results showed that the optimum conditions were 45ºC and pH 5.5. Optimal stability and reusability of the immobilized transglucosidase were obtained when 0.5% EDC was used as the crosslinker. In addition, more than 80% on a dry weight basis of IMO was obtained, the immobilized dual biocatalyst worked for the production of high-content IMO from maltose.
Advisor Committee Dey-Chyi Sheu - advisor
none - co-chair
none - co-chair
Files Date of Defense 2010-07-26 Date of Submission 2010-08-31