首頁 > 網路資源 > 大同大學數位論文系統

Title page for etd-0831111-133947


URN etd-0831111-133947 Statistics This thesis had been viewed 1520 times. Download 0 times.
Author Shuo-Ming Hsu
Author's Email Address No Public.
Department Bioengineering
Year 2010 Semester 2
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 127
Title Identification of proanthocyanidin composition from Bischofia javanica Blume
Keyword
  • Bischofia javanic
  • antioxidant
  • proanthocyanidin
  • proanthocyanidin
  • antioxidant
  • Bischofia javanic
  • Abstract Aqueous fraction of methanolic extract from Bischofia javanica as reported to be rich in proanthocyanidin with high antioxidant activity. In this study, it was chromatographed on Sephadex LH-20 column into seven subfractions. M1、M3 and A3 subfractions exhibited strong DPPH radical scavenging activities, while M1 subfraction had the highest proanthocyanidin content. M1 subfraction was thiolyzed with toluene-?-thio, followed by LC-MS/MS, then the thiolyed productes was collected by semi-preparative HPLC, followed by HRMS resolution. Results showed that the polymeric proanthocyanidins were highly heterogeneous. (+)Catechin, (-)epicatechin gallate and (-)epigallocatechin gallate were the main terminal units, while catechin, gallocatechin, catechin gallate and gallocatechin gallate were as extension units. Through examining the thiolyzed products by HPLC-PDA, it was found that the terminal units and extension units of each subfraction, except M2, were identical to those of M1 subfraction. The main extension unit of each subfraction was catechin. Pearson correlation analysis suggested that the DPPH radical scavenging activity of subfractions was highly correlated with the molar percentage of galloylation (G %), but not (with) the mean degree of polymerization (mDP).
    Advisor Committee
  • Shiow-Ling Lee - advisor
  • Chi-Yang Yu - co-chair
  • Lie-Chwen Lin - co-chair
  • Files indicate in-campus access at 5 years and off-campus not accessible
    Date of Defense 2011-07-28 Date of Submission 2011-08-31


    Browse | Search All Available ETDs