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URN etd-0901111-084946 Statistics This thesis had been viewed 2594 times. Download 990 times. Author Bo-Hong Jiang Author's Email Address firstname.lastname@example.org Department Bioengineering Year 2010 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 107 Title Ethanol production from carrot pomace using thermotolerant yeasts Kluyveromyces marxianus Keyword Kluyveromyces marxianus Pectinase ethanol fermentation AccelleraseTM 1000 carrot pomace carrot pomace AccelleraseTM 1000 ethanol fermentation Pectinase Kluyveromyces marxianus Abstract The juice industry in Taiwan produces large amount of agricultural wastes. Carrot pomace, which is the subject of present study, is one common example. Traditionally, carrot pomace is used as pasture feed or fertilizer. In carrot pomace, lignin composes only a small fraction of lignocellulose, the major components of lignocellulose being cellulose and hemicellulose, which are easier for enzymatic saccharification than lignin.
Two enzymes (AccelleraseTM 1000 and Pectinase) were found to hydrolyze 45% ~ 50% (w/w) of carrot pomace and the major hydrolysis products were glucose and galactose.
Simultaneous Saccharification and Fermentation (SSF) at 42℃ was performed on 10% (w/v) carrot pomace and the process produced 18 g/L ethanol. The yield is 18% (g-ethanol/g-carrot pomace). Under fed-batch of additional 5% (w/v) of substrate, 30 g/L ethanol was produced. The yield is 20% (g-ethanol/g-carrot pomace). The further addition of ten percent carrot pomace would lead to ethanol fermentation and produce 37 g/L of ethanol. Due to the fact that no pre-treatment is required for carrot pomace, it is capable of manufacturing ethanol directly, thus reducing capital cost for developing alternative biomass energy.
Advisor Committee Chiyang Yu - advisor
Kow-Jen Duan - advisor
Hong-Wei Yen - co-chair
Files Date of Defense 2011-07-28 Date of Submission 2011-09-01